全面评述毛细管电泳-质谱法在推进生物分子研究中的应用。

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2024-11-07 DOI:10.1002/elps.202400122

Laura Pont, María Vergara-Barberán, Enrique Javier Carrasco-Correa

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引用次数: 0

摘要

本综述深入探讨了毛细管电泳-质谱法（CE-MS）在 2020 至 2024 年生物分子研究中的应用。毛细管电泳质谱（CE-MS）具有众多优势，是一种用途广泛的强大工具，有助于分析各种生物大分子，包括蛋白质、肽、寡核苷酸和其他代谢物，如脂类、碳水化合物或胺等。该综述涵盖了 CE-MS 耦合的各种 CE 模式和界面，为其在生物分子研究中的应用提供了全面的见解。此外，它还有效地总结了 CE-MS 中使用的条件，同时还涉及到样品制备要求等关键方面。尽管 CE-MS 具有诸多优势，但该综述强调了发现与实际应用之间的差距，强调了大规模验证和方法标准化的必要性，以充分发挥 CE-MS 在生物分子研究中的潜力。

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A Comprehensive Review on Capillary Electrophoresis-Mass Spectrometry in Advancing Biomolecular Research.

This review provides an in-depth exploration of capillary electrophoresis-mass spectrometry (CE-MS) in biomolecular research from 2020 to 2024. CE-MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites, such as lipids, carbohydrates, or amines, among others. The review extends to various CE modes and interfaces for the CE-MS coupling, offering comprehensive insights into their applications within biomolecular research. Furthermore, it effectively summarizes the conditions employed in CE-MS while also addressing critical aspects such as sample preparation requirements. Despite its advantages, the review highlights a gap between discovery and practical implementation, underscoring the need for large-scale validation and method standardization to fully realize the potential of CE-MS in biomolecular research.

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.