一种新型纳米羟基磷灰石琼脂糖水凝胶用于脱矿化人类珐琅质的仿生再矿化:体外研究

IF 1.5 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Clinical, Cosmetic and Investigational Dentistry Pub Date : 2024-11-02 eCollection Date: 2024-01-01 DOI:10.2147/CCIDE.S478045
Sara El Moshy, Israa Ahmed Radwan, Manal Matoug-Elwerfelli, Ahmed Abdou, Marwa M S Abbass
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引用次数: 0

摘要

目的:本研究旨在探讨琼脂糖水凝胶单独或与纳米羟基磷灰石(n-HA-EMD-琼脂糖)联合负载釉质基质衍生物(EMD-琼脂糖)对人类脱矿釉质再矿化的生物模拟作用:方法:将拔出的人类下颌第三磨牙切成 54 个颊舌半部分。除两个珐琅质窗口外,每一半都涂上了耐酸指甲油。对釉质表面的微硬度、能量色散 X 射线光谱(EDX)和扫描电子显微镜(SEM)进行了分析,以评估釉质表面的基线、37% 磷酸脱矿化后以及每次水凝胶应用和再矿化 2 天、4 天和 6 天后的情况。再矿化是在 37°C 温度下使用磷酸盐溶液进行的:在再矿化后的第 6 天,n-HA-EMD-琼脂糖水凝胶的平均显微硬度(260.87 ± 3.52)与 EMD-琼脂糖水凝胶(244.63 ± 2.76)相比具有显著的统计学意义(p = 0.027)。同样,与 EMD-琼脂糖水凝胶钙(19.64 ± 1.092)、磷(19.64 ± 1.092)和氟(0.7033 ± 0.0624)重量百分比相比,n-HA-EMD-琼脂糖水凝胶显示出更高的平均钙(46.31 ± 2.78)、磷(24.92 ± 0.826)和氟(0.909 ± 0.053)重量百分比(p < 0.05)。此外,扫描电子显微镜分析表明,在使用 n-HA-EMD-agarose 水凝胶后,n-HA 大量沉积,而 EMD-agarose 则显示出相对光滑的珐琅质表面,矿物质沉积导致的珐琅质棒状物不明显:结论:n-HA-EMD-琼脂糖水凝胶组合改善了再矿化珐琅质的表面微硬度,提高了矿物质含量沉积,表明其具有作为牙釉质修复生物仿生方法的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel Nano-Hydroxyapatite Agarose-Based Hydrogel for Biomimetic Remineralization of Demineralized Human Enamel: An in-vitro Study.

Purpose: This study aims to investigate the biomimetic effect of agarose hydrogel loaded with enamel matrix derivative (EMD-agarose) alone or in combination with nano-hydroxyapatite (n-HA-EMD-agarose) on the remineralization of human demineralized enamel.

Methods: Extracted human mandibular third molars were sectioned into 54 buccal and lingual halves. Acid-resistant nail varnish was applied to each half, except for two enamel windows. Enamel surface microhardness, energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM) analyses were conducted to evaluate enamel surfaces at baseline, following demineralization with 37% phosphoric acid, and after each hydrogel application and remineralization for two, four, and six days. Remineralization was performed using a phosphate solution at 37°C.

Results: At day 6 following remineralization, a statistically significant higher mean microhardness was recorded in n-HA-EMD-agarose hydrogel (260.87 ± 3.52) as compared to EMD-agarose hydrogel (244.63 ± 2.76) (p = 0.027). Similarly, n-HA-EMD-agarose hydrogel showed a higher mean calcium (46.31 ± 2.78), phosphorous (24.92 ± 0.826), and fluoride (0.909 ± 0.053) weight percentage compared to EMD-agarose hydrogel calcium (19.64 ± 1.092), phosphorous (19.64 ± 1.092), and fluoride (0.7033 ± 0.0624) weight percentage (p < 0.05). Further, SEM analysis revealed a substantial deposition of n-HA following the application of the n-HA-EMD-agarose hydrogel, whereas the EMD-agarose exhibited a relatively smooth enamel surface with less visible enamel rods due to mineral deposition.

Conclusion: The combined n-HA-EMD-agarose hydrogel demonstrated improved surface microhardness of the remineralized enamel and enhanced mineral content deposition, indicating its potential as a biomimetic approach for dental enamel repair.

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来源期刊
CiteScore
3.90
自引率
5.60%
发文量
43
审稿时长
16 weeks
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