COL11A1在泛癌症中致癌作用的生物信息学分析和实验验证。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI:10.1007/s13205-024-04133-0
Xiaofeng Wan, Qingmei Deng, Anling Chen, Xinhui Zhang, Wulin Yang
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引用次数: 0

摘要

COL11A1在不同癌症类型中错综复杂的表达模式和致癌属性在很大程度上仍然难以捉摸。本研究利用几个公共数据库(TCGA、GTEx 和 CCLE)研究了 COL11A1 的泛癌症表达、其预后影响、与免疫微环境的相互作用以及丰富的信号级联。同时,还进行了 Western 印迹分析,以验证 COL11A1 在肺腺癌(LUAD)细胞系和临床样本中的表达。此外,研究人员还生成了 COL11A1 基因敲除细胞系,通过 MTT、菌落形成和划痕伤口愈合试验来研究 COL11AI 表达对癌细胞行为的功能性影响。一项全面的数据库调查显示,COL11A1在大多数肿瘤组织中上调,其表达与患者的预后高度相关。值得注意的是,COL11A1的基因改变主要表现为突变,而其DNA甲基化状态与多个启动子区域的基因表达水平成反比。我们的研究结果表明,COL11A1有助于调节肿瘤免疫格局,并可能通过上皮-间质转化(EMT)途径发挥其致癌功能。Western印迹分析进一步证实了COL11A1在LUAD细胞系和组织中的特异性上调,表明它与EMT过程密切相关。在癌细胞中消减 COL11A1 能明显降低其增殖、克隆和迁移能力,凸显了 COL11A1 在肿瘤细胞行为中的功能意义。总之,这项研究揭示了 COL11A1 在泛癌症组织中的普遍过表达、其与预后和微环境的密切关系,以及其通过 EMT 信号介导的肿瘤促进作用的机理基础。我们的研究结果表明,COL11A1 可作为癌症的预后和诊断生物标志物及治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioinformatics analysis and experimental validation of the oncogenic role of COL11A1 in pan-cancer.

The intricate expression patterns and oncogenic attributes of COL11A1 across different cancer types remain largely elusive. This study used several public databases (TCGA, GTEx, and CCLE) to investigate the pan-cancer landscape of COL11A1 expression, its prognostic implications, interplay with the immune microenvironment, and enriched signaling cascades. Concurrently, western blot analyses were performed to verify COL11A1 expression in lung adenocarcinoma (LUAD) cell lines and clinical samples. In addition, COL11A1 knockout cell lines were generated to scrutinize the functional consequences of COL11AI expression on cancer cell behavior by use MTT, colony formation, and scratch wound healing assays. A comprehensive database investigation revealed that COL11A1 was upregulated in a majority of tumor tissues and its expression was highly correlated with a patient's prognosis. Notably, genetic alterations in COL11A1 predominantly occurred as mutations, while its DNA methylation status inversely mirrored gene expression levels across multiple promoter regions. Our findings suggest that COL11A1 helps to modulate the tumor immune landscape and potentially acts through the epithelial-mesenchymal transition (EMT) pathway to exert its oncogenic function. Western blot analyses further substantiated the specific upregulation of COL11A1 in LUAD cell lines and tissues, suggesting a close association with the EMT process. Ablation of COL11A1 in cancer cells significantly reduced their proliferative, clonogenic, and migratory abilities, underscoring the functional significance of COL11A1 in tumor cell behavior. Collectively, this research revealed the prevalent overexpression of COL11A1 in pan-cancer tissues, its profound prognostic and microenvironmental correlations, and the mechanistic underpinnings of its tumor-promoting effects as mediated via EMT signaling. Our findings suggest that COL11A1 could serve as a prognostic and diagnostic biomarker and therapeutic target for cancer.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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