利用腊菊水提取物绿色合成的氧化锌纳米颗粒的抗糖尿病、抗炎、抗氧化和细胞毒性潜力。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI:10.1007/s13205-024-04125-0
Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju
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引用次数: 0

摘要

目前的研究涉及利用腊菊嫩枝的水提取物合成氧化锌纳米颗粒(ZnO-NPs),并随后通过不同的分析方法进行表征,如紫外可见光谱、扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDX)、X 射线衍射(XRD)、透射电子显微镜(TEM)和 zeta 电位。然后,通过一系列分析,包括细胞毒性、抗氧化、抗炎和酶抑制抗糖尿病作用,测试了 ZnO-NPs 对 C3A 肝细胞和 L6 肌细胞的生物效应。紫外可见光谱分析显示,该化合物在 360 波长处有最大吸收光谱;TEM 分析显示,该化合物呈球形和六边形结构,平均尺寸为 28.05-58.3 nm;XRD 显示,该化合物呈结晶六边形结构。zeta电位评估表明,ZnO-NPs在- 20 mV下相对稳定,傅立叶变换红外光谱分析确定了一些与酚类、羧酸和酰胺相关的重要官能团,它们是还原和稳定ZnO-NPs的主要成分。合成的 ZnO-NPs 在较高浓度(125 微克/毫升和 250 微克/毫升)时对细胞系有细胞毒性作用,这使得炎症和抗氧化检测结果的解释变得复杂。然而,在较高浓度下(125 微克/毫升和 250 微克/毫升),锰氧化物对细胞株的细胞毒性作用明显(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum.

The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of Helichrysum cymosum shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (p < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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