Hongying Jia , Nuo Zhang , Yuyang Li , Hongmin Ma , Dan Wu , Xuejing Liu , Huangxian Ju , Qin Wei
{"title":"基于自增强 Ce(III, IV)-MOF@Ru 到 CuO@PDA@AuNPs 的共振能量转移的多淬灭电化学发光系统,用于 HER2 的超灵敏检测","authors":"Hongying Jia , Nuo Zhang , Yuyang Li , Hongmin Ma , Dan Wu , Xuejing Liu , Huangxian Ju , Qin Wei","doi":"10.1016/j.snb.2024.136905","DOIUrl":null,"url":null,"abstract":"<div><div>This study presents an innovative approach based on electrochemiluminescence resonance energy transfer (ECL-RET) through the introduction of a new pattern donor-acceptor couple. The donor of self-enhanced Ce(III, IV)-MOF@Ru is created by immobilizing Ru(bpy)<sub>3</sub><sup>2+</sup> on Ce-based metal-organic frameworks (Ce(III, IV)-MOF). The acceptor of CuO@PDA@AuNPs is the CuO nanospheres polydopamine (PDA) framework grafted with gold nanoparticles (AuNPs). An ultrasensitive detection of human epidermal growth factor receptor-2 (HER2) was achieved through the development of a quenched ECL immunosensor. Ce(III, IV)-MOF, a unique 3D infinite extension framework, was recognized for its excellent nanostructure and remarkable capacity to greatly activate tripropylamine (TPrA) and generate abundant radicals. Fortunately, it was simultaneously utilized as a highly effective coreactant accelerator and encapsulation agent, thereby facilitating the immobilization of Ru(bpy)<sub>3</sub><sup>2+</sup> and the generation of radicals for creating a self-enhanced emitter of Ce(III, IV)-MOF@Ru. Consequently, by effectively reducing the distance of electron transmission and minimizing the loss of energy, Ce(III, IV)-MOF@Ru achieved a significantly high efficiency in ECL. More importantly, CuO@PDA@AuNPs was prepared as a perfect quenching agent. The ultraviolet-visible (UV–vis) spectra of CuO@PDA@AuNPs exhibited partial overlap with ECL spectra of Ce(III, IV)-MOF@Ru, thus efficiently initiating the ECL-RET interaction between the donor and acceptor. With the purpose of demonstrating the superiority of newly obtained self-enhanced nanoemitter and donor-acceptor couple, an ECL immunoassay was proposed for the analysis of HER2 with range of 0.2 fg/mL ∼ 10 ng/mL and the limit of detection of 0.067 fg/mL. Therefore, this method supplies convenient and significant strategy for the clinical analysis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136905"},"PeriodicalIF":8.0000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multi-quenching electrochemiluminescence system based on resonance energy transfer from self-enhanced Ce(III, IV)-MOF@Ru to CuO@PDA@AuNPs for ultrasensitive detection of HER2\",\"authors\":\"Hongying Jia , Nuo Zhang , Yuyang Li , Hongmin Ma , Dan Wu , Xuejing Liu , Huangxian Ju , Qin Wei\",\"doi\":\"10.1016/j.snb.2024.136905\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study presents an innovative approach based on electrochemiluminescence resonance energy transfer (ECL-RET) through the introduction of a new pattern donor-acceptor couple. The donor of self-enhanced Ce(III, IV)-MOF@Ru is created by immobilizing Ru(bpy)<sub>3</sub><sup>2+</sup> on Ce-based metal-organic frameworks (Ce(III, IV)-MOF). The acceptor of CuO@PDA@AuNPs is the CuO nanospheres polydopamine (PDA) framework grafted with gold nanoparticles (AuNPs). An ultrasensitive detection of human epidermal growth factor receptor-2 (HER2) was achieved through the development of a quenched ECL immunosensor. Ce(III, IV)-MOF, a unique 3D infinite extension framework, was recognized for its excellent nanostructure and remarkable capacity to greatly activate tripropylamine (TPrA) and generate abundant radicals. Fortunately, it was simultaneously utilized as a highly effective coreactant accelerator and encapsulation agent, thereby facilitating the immobilization of Ru(bpy)<sub>3</sub><sup>2+</sup> and the generation of radicals for creating a self-enhanced emitter of Ce(III, IV)-MOF@Ru. Consequently, by effectively reducing the distance of electron transmission and minimizing the loss of energy, Ce(III, IV)-MOF@Ru achieved a significantly high efficiency in ECL. More importantly, CuO@PDA@AuNPs was prepared as a perfect quenching agent. The ultraviolet-visible (UV–vis) spectra of CuO@PDA@AuNPs exhibited partial overlap with ECL spectra of Ce(III, IV)-MOF@Ru, thus efficiently initiating the ECL-RET interaction between the donor and acceptor. With the purpose of demonstrating the superiority of newly obtained self-enhanced nanoemitter and donor-acceptor couple, an ECL immunoassay was proposed for the analysis of HER2 with range of 0.2 fg/mL ∼ 10 ng/mL and the limit of detection of 0.067 fg/mL. Therefore, this method supplies convenient and significant strategy for the clinical analysis.</div></div>\",\"PeriodicalId\":425,\"journal\":{\"name\":\"Sensors and Actuators B: Chemical\",\"volume\":\"424 \",\"pages\":\"Article 136905\"},\"PeriodicalIF\":8.0000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors and Actuators B: Chemical\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0925400524016356\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925400524016356","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Multi-quenching electrochemiluminescence system based on resonance energy transfer from self-enhanced Ce(III, IV)-MOF@Ru to CuO@PDA@AuNPs for ultrasensitive detection of HER2
This study presents an innovative approach based on electrochemiluminescence resonance energy transfer (ECL-RET) through the introduction of a new pattern donor-acceptor couple. The donor of self-enhanced Ce(III, IV)-MOF@Ru is created by immobilizing Ru(bpy)32+ on Ce-based metal-organic frameworks (Ce(III, IV)-MOF). The acceptor of CuO@PDA@AuNPs is the CuO nanospheres polydopamine (PDA) framework grafted with gold nanoparticles (AuNPs). An ultrasensitive detection of human epidermal growth factor receptor-2 (HER2) was achieved through the development of a quenched ECL immunosensor. Ce(III, IV)-MOF, a unique 3D infinite extension framework, was recognized for its excellent nanostructure and remarkable capacity to greatly activate tripropylamine (TPrA) and generate abundant radicals. Fortunately, it was simultaneously utilized as a highly effective coreactant accelerator and encapsulation agent, thereby facilitating the immobilization of Ru(bpy)32+ and the generation of radicals for creating a self-enhanced emitter of Ce(III, IV)-MOF@Ru. Consequently, by effectively reducing the distance of electron transmission and minimizing the loss of energy, Ce(III, IV)-MOF@Ru achieved a significantly high efficiency in ECL. More importantly, CuO@PDA@AuNPs was prepared as a perfect quenching agent. The ultraviolet-visible (UV–vis) spectra of CuO@PDA@AuNPs exhibited partial overlap with ECL spectra of Ce(III, IV)-MOF@Ru, thus efficiently initiating the ECL-RET interaction between the donor and acceptor. With the purpose of demonstrating the superiority of newly obtained self-enhanced nanoemitter and donor-acceptor couple, an ECL immunoassay was proposed for the analysis of HER2 with range of 0.2 fg/mL ∼ 10 ng/mL and the limit of detection of 0.067 fg/mL. Therefore, this method supplies convenient and significant strategy for the clinical analysis.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.