微生物生产增值天然产品的代谢工程最新趋势

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-10-21 DOI:10.1016/j.bej.2024.109537

Prasenjit Chakraborty , Randhir Kumar , Sanjay Karn , Payal Patel , Haren Gosai

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引用次数: 0

摘要

以微生物为媒介的化学生产是一种生产对社会十分重要的天然产品的可持续方法。药物、营养保健品、色素、芳香化合物和生物燃料等天然产品的传统发酵生产具有产量低、成本高和耗时长等局限性。通过代谢工程改变微生物的代谢途径，在经济生产这些天然产品方面取得了巨大成功。新工具和新策略的最新进展大大缩短了传统方法（即代谢工程中的设计-构建-测试-学习（DBTL）循环）的周转时间。在此，我们总结了代谢工程领域开发的各种工具和策略，重点关注增值天然产品。此外，本综述还列举了过去五年中通过代谢工程对微生物进行生物勘探以生产天然产品的情况。最后，报告了成功建立强大的工程微生物细胞工厂的前景、挑战和机遇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Recent trends in metabolic engineering for microbial production of value-added natural products

Microbe-mediated chemical production is a sustainable approach for production of natural products that are important for our society. Traditional fermentation production of natural products viz. drugs, nutraceuticals, pigments, aromatic compounds, and biofuels has limitations such as low yield, high cost and time- consuming process. Significant success in the economical production of these natural products has been established by altering the metabolic pathways of microorganisms through metabolic engineering. Recent advancements in new tools and strategies have led to a significant reduction in the turnaround time in the classic approach i.e. design-build-test-learn (DBTL) cycle in metabolic engineering. Here, we summarize various tools and strategies developed in the area of metabolic engineering with a focus on value-added natural products Also, this review lists bioprospecting of microorganisms through metabolic engineering in last five years for production of natural products. At the end, outlook, challenges and opportunities for the successful establishment of robust engineered microbial cell factories have been reported.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.