{"title":"ets-10p::gfp 的表达可预测 daf-16; daf-7 幼虫的矮小症形成。","authors":"Juanez T Lindsay, Matthew J Wirick, Xantha Karp","doi":"10.17912/micropub.biology.001358","DOIUrl":null,"url":null,"abstract":"<p><p>In adverse conditions, <i>Caenorhabditis elegans</i> larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog <i>daf-16</i> promotes dauer formation, but <i>daf-16</i> mutants can enter dauer with incomplete penetrance in combination with a mutation in <i>daf-7</i> /TGFβ. The degree to which <i>daf-16 ; daf-7</i> larvae enter L2d is unknown. Here we show that many <i>daf-16 ; daf-7</i> mutants express intermediate levels of the <i>ets-10p::gfp</i> L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of <i>ets-10p::gfp</i> expression early in the second larval stage partially predicts which <i>daf-16 ; daf-7</i> larvae will bypass dauer formation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536051/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>ets-10p::gfp</i> expression is predictive of dauer formation in <i>daf-16; daf-7</i> larvae.\",\"authors\":\"Juanez T Lindsay, Matthew J Wirick, Xantha Karp\",\"doi\":\"10.17912/micropub.biology.001358\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In adverse conditions, <i>Caenorhabditis elegans</i> larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog <i>daf-16</i> promotes dauer formation, but <i>daf-16</i> mutants can enter dauer with incomplete penetrance in combination with a mutation in <i>daf-7</i> /TGFβ. The degree to which <i>daf-16 ; daf-7</i> larvae enter L2d is unknown. Here we show that many <i>daf-16 ; daf-7</i> mutants express intermediate levels of the <i>ets-10p::gfp</i> L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of <i>ets-10p::gfp</i> expression early in the second larval stage partially predicts which <i>daf-16 ; daf-7</i> larvae will bypass dauer formation.</p>\",\"PeriodicalId\":74192,\"journal\":{\"name\":\"microPublication biology\",\"volume\":\"2024 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536051/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"microPublication biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17912/micropub.biology.001358\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001358","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
ets-10p::gfp expression is predictive of dauer formation in daf-16; daf-7 larvae.
In adverse conditions, Caenorhabditis elegans larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog daf-16 promotes dauer formation, but daf-16 mutants can enter dauer with incomplete penetrance in combination with a mutation in daf-7 /TGFβ. The degree to which daf-16 ; daf-7 larvae enter L2d is unknown. Here we show that many daf-16 ; daf-7 mutants express intermediate levels of the ets-10p::gfp L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of ets-10p::gfp expression early in the second larval stage partially predicts which daf-16 ; daf-7 larvae will bypass dauer formation.
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