Sheida Alizadeh, Fahime Edalat, Arash Letafati, Neda Pirbonyeh, Alireza Tabibzadeh, Leila Mousavizadeh, Afagh Moattari, Mohammad Hadi Karbalaie Niya
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引用次数: 0
摘要
流感病毒神经氨酸酶抑制剂(NAI)药物的使用会导致NAI耐药,尤其是在儿童和免疫系统较弱的人群中。本研究旨在确定 IBV 的 NAI 耐药变体,并根据伊朗设拉子患者的 NA 基因介绍可能的新型突变、系统发育研究及其表位图谱。2017 年至 2018 年期间,对有症状的儿童进行了横断面研究。对 IBV 进行了实时 PCR 筛查。然后,利用直接测序技术扩增了 NA 基因的 1401 个碱基,并重建了系统发生树。使用 ABCpred 服务器预测表位。在总共 235 份标本中,9.7% 被确定感染了 IBV。对其中 17 个分离株的 NA 基因序列进行了分析。系统发生学分析表明,15 个分离株属于山形支系 3 Wisconsin/01-like 亚支系,2 个与维多利亚支系 1 Brisbane/60-like 亚支系(Vic-1A-2)有关。NA 基因序列分析表明共有 52 个替换,其中 27 个为 BVic 分离物的替换,37 个为 BYam 分离物的替换,19 个为新替换。在 NA 活性位点只发现了一个取代(S198N),T49M、I120V、N198S、N219K、S295R、D320K、N340D、E358K、D384G 和 D463N 被认为是可能对 NAIs 产生抗性的变体。外显子图谱显示,我们分离的 NA 基因存在一些重大差异。本研究是设拉子/伊朗就 IBV 对 NAIs 的耐药相关变异进行的罕见的综合性研究之一。我们报告了11.7%的NA活性位点变异和一些可能对NAIs耐药的变异。表位图谱证实了NA基因的重大变化,这需要更广泛的研究来证实。
Genetic characterization of influenza B virus and oseltamivir resistance in pediatric patients with acute respiratory infections: a cross-sectional study.
Influenza virus neuraminidase inhibitors (NAIs) drug usage can result in NAI resistance, especially in children and individuals with weakened immune systems. The aim of the present study was to identify NAI-resistant variants of IBV and to introduce probable novel mutations, phylogenetic study, and its epitope mapping based on NA gene in patients from Shiraz, Iran. A cross-sectional study was conducted between 2017 and 2018 on symptomatic children. A real-time PCR was run for IBV screening. Then, making use of direct sequencing, amplified 1401 bases of NA gene and phylogenetic tree reconstructed. Epitopes were predicted using ABCpred server. From among a total of 235 specimens, 9.7% were identified with IBV infection. Of them, sequence of NA gene for 17 isolates were analyzed. Phylogenetic analysis showed that 15 isolates belonged to Yamagata clade 3 Wisconsin/01-like subclade and 2 were related to Victoria clade 1 Brisbane/60-like subclade (Vic-1A-2). NA gene sequence analysis showed a total of 52 substitutions in which 27 were for BVic and 37 were for BYam isolates and 19 were novel substitutions. Only one substitution (S198N) was found in NA active site and T49M, I120V, N198S, N219K, S295R, D320K N340D, E358K, D384G, and D463N were found as probable resistance variants to NAIs. Epitope mapping showed some major differences in our isolates NA gene. Present study was one of the rare comprehensive studies conducted in Shiraz/Iran on IBV resistant associated variants to NAIs. We reported 11.7% mutation in NA active site and some probable NAIs resistant mutations. Epitope mapping confirmed major changes in NA gene which needs broader studies to confirm.
期刊介绍:
Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools.
Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments.
Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.