StCDF1:万能 "时钟输出,在调节马铃薯硝酸盐还原活性方面发挥核心作用。

IF 9.4 1区 生物学 Q1 Agricultural and Biological Sciences
New Phytologist Pub Date : 2024-11-06 DOI:10.1111/nph.20186
Maroof Ahmed Shaikh, Lorena Ramírez-Gonzales, José M Franco-Zorrilla, Evyatar Steiner, Marian Oortwijn, Christian W B Bachem, Salomé Prat
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引用次数: 0

摘要

CYCLING DOF FACTOR(CDF)家族的转录因子可激活马铃薯叶片中的SP6A FT块茎化信号。在现代栽培品种中,截短的StCDF1.2等位基因通过稳定StCDF1蛋白而超越了严格的SD控制,从而导致StCOL1受抑制,并削弱了反凝固性SP5G旁系亲属的激活。通过DAP-seq和RNA-seq研究,我们发现StCDF1不仅是昼长通路的上游调控因子,还直接调控多个氮同化和转运基因。StCDF1 直接抑制硝酸盐还原酶(NR/NIA)的表达,该酶催化硝酸盐同化作用中的第一个还原步骤,由一个马铃薯基因座编码。StCDF1基因敲除株系在硝酸盐限制条件下表现更好,这种表型与StNR表达受抑制有关。此外,StNR DAP-seq区域的缺失也消除了StCDF1的抑制作用,但并不影响NLP7对StNR启动子的依赖性激活。我们在具有早期StCDF1等位基因的马铃薯栽培品种中发现了DAP-seq区域的多个核苷酸多态性,这表明这种遗传变异是作为StCDF1稳定的负面影响的补偿机制而被选择的。因此,对 StCDF1 识别元件进行定向修饰是提高马铃薯中 StNR 限制性活性的一种有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
StCDF1: A 'jack of all trades' clock output with a central role in regulating potato nitrate reduction activity.

Transcription factors of the CYCLING DOF FACTOR (CDF) family activate in potato the SP6A FT tuberization signal in leaves. In modern cultivars, truncated StCDF1.2 alleles override strict SD control by stabilizing the StCDF1 protein, which leads to StCOL1 suppression and impaired activation of the antagonic SP5G paralog. By using DAP-seq and RNA-seq studies, we here show that StCDF1 not only acts as an upstream regulator of the day length pathway but also directly regulates several N assimilation and transport genes. StCDF1 directly represses expression of NITRATE REDUCTASE (NR/NIA), which catalyses the first reduction step in nitrate assimilation, and is encoded by a single potato locus. StCDF1 knock-down lines performed better in N-limiting conditions, and this phenotype correlated with derepressed StNR expression. Also, deletion of the StNR DAP-seq region abolished repression by StCDF1, while it did not affect NLP7-dependent activation of the StNR promoter. We identified multiple nucleotide polymorphisms in the DAP-seq region in potato cultivars with early StCDF1 alleles, suggesting that this genetic variation was selected as compensatory mechanism to the negative impact of StCDF1 stabilization. Thereby, directed modification of the StCDF1-recognition elements emerges as a promising strategy to enhance limiting StNR activity in potato.

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来源期刊
New Phytologist
New Phytologist PLANT SCIENCES-
CiteScore
17.60
自引率
5.30%
发文量
728
审稿时长
1 months
期刊介绍: New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.
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