嗜中性沙门氏菌的特征以及用于区分非典型菌株和典型菌株的 PCR 的开发。

IF 2.2 3区农林科学 Q2 FISHERIES

Journal of fish diseases Pub Date : 2024-11-06 DOI:10.1111/jfd.14028

Teresa Wilson, Mark Green, Vivianne Dunn, David Cummins, Matthew Neave

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引用次数: 0

摘要

本研究描述了从塔斯马尼亚州的大西洋鲑鱼（Salmo salar L.）中分离出的一种新的嗜中性鲑鱼单胞菌（Aeromonas salmonicida）菌株的鉴定和特征描述，该菌株被命名为 HMes1。通过表型、系统发育和遗传特征鉴定，分离物被确定为嗜盐单胞菌。表型鉴定后，对诊断表型鉴定系统 MicroSys A24 进行了更新，并开发了一种新的多重常规 PCR，以快速、低成本地鉴定非典型鲑单胞菌，并排除外来菌株鲑单胞菌（A. salmonicida ssp. salmonicida）。

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Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains

This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.

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来源期刊

Journal of fish diseases 农林科学-海洋与淡水生物学

CiteScore

4.60

自引率

12.00%

发文量

170

审稿时长

6 months

期刊介绍： Journal of Fish Diseases enjoys an international reputation as the medium for the exchange of information on original research into all aspects of disease in both wild and cultured fish and shellfish. Areas of interest regularly covered by the journal include: -host-pathogen relationships- studies of fish pathogens- pathophysiology- diagnostic methods- therapy- epidemiology- descriptions of new diseases