用于活细胞信息存储和生物成像的便携式 Zn2+ 荧光传感器

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2024-11-05 DOI:10.1007/s10895-024-04018-8

Xiujuan Li, Tinghuan Gao, Shoucheng Wang, Qing Zhang, Siyu Chen, Hongshuo Zhang, Jinbin Xu, Xinchao Wang, Qianqian Liu

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引用次数: 0

摘要

基于激发态分子内质子转移（ESIPT）机制，设计并合成了一种用于快速检测 Zn2+ 的荧光探针（Probe-ITR）。利用紫外-可见光谱和荧光发射光谱测试了 Probe-ITR 对 Zn2+ 的特异性识别能力。结果表明，在目标探针分子的混合溶液（DMSO/PBS = 5/5）中加入适量的 Zn2+ 后，40 秒内就能产生快速反应。在 365 纳米紫外线照射下，无色溶液变为黄绿色荧光，强度增加了 150 倍。此外，探针分子对 Zn2+ 的特异性识别检测限仅为 17.3 nmol/L，灵敏度极高。通过信息存储和细胞成像实验，探针分子的实际应用潜力得到了提高。

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A Portable Zn²⁺ Fluorescence Sensor for Information Storage and Bio-Imaging in Living Cells.

A fluorescence probe (Probe-ITR) was designed and synthesized for the rapid detection of Zn²⁺ based on the excited state intramolecular proton transfer (ESIPT) mechanism. The specific recognition ability of Probe-ITR to Zn²⁺ was tested using UV-VIS and fluorescence emission spectroscopy. The results demonstrated a rapid response within 40s upon addition of an appropriate amount of Zn²⁺ into the mixed solution of the target probe molecules (DMSO/PBS = 5/5). Under 365 nm ultraviolet light irradiation, the colorless solution changed to yellow-green fluorescence with a 150-folds increase in intensity. Furthermore, the detection limit for specific recognition of Zn²⁺ by the probe molecule is only 17.3 nmol/L, indicating high sensitivity. The practical application potential of the probe molecules was enhanced by employing on information storage and conducting cell imaging experiments.

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.