在雄性 Sprague-Dawley 大鼠体内补充维生素 D3 和进行高强度间歇训练可改善果糖诱导的肝脏脂质积累。

IF 3.9 2区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Behnaz Shokri, Hamid Mohebbi, Javad Mehrabani
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引用次数: 0

摘要

背景:肝内脂质蓄积(IHL)是代谢紊乱的标志,与新生脂肪生成(DNL)密切相关。值得注意的是,果糖喂养会增加 DNL。通过改变饮食和增加体力活动/锻炼来调整生活方式可降低 IHL 含量。我们研究了维生素 D3 补充剂 (VDS)、高强度间歇训练 (HIIT) 及其组合对喂食高果糖饮食 (HFrD) 的雄性 Sprague-Dawley 大鼠 DNL 途径转录因子和酶的影响:方法:将 40 只雄性大鼠分为 5 组(n = 8):方法:将 40 只雄性大鼠分为 5 组(n = 8):CS 组(对照组采用标准饮食);CF 组(对照组采用 HFrD(10%(w/v)自来水中的果糖溶液));FT 组(HFrD + HIIT:FD(HFrD + 腹腔注射 10000 IU/kg/week VDS 干预);FTD(HFrD + HIIT + VDS),维持 12 周。采用ELISA、GOD-POD检测、folch、Western印迹和油红O染色法分别测定胰岛素、空腹血糖(FBG)、肝甘油三酯(TG)和胆固醇水平;SREBP1c、ChREBP-β、ACC1、FASN、p-ACC1、AMPK、p-AMPK和PKA蛋白表达以及IHL含量:结果:HIIT 和 VDS 都能显著提高 PKA、AMPK、p-AMPK 和 p-ACC1 的水平,同时显著降低 SREBP1c、ChREBP-β、ACC1、FASN、胰岛素、FBG、肝脏 TG、肝脏胆固醇和 IHL 的水平。在降低 ChREBP-β、ACC1、胰岛素、FBG、肝脏 TG 和胆固醇以及增加 p-ACC1 和 PKA 方面,HIIT 比 VDS 表现出更好的效果。值得注意的是,HIIT 和 VDS 联合干预对所有参数的改善最为显著:结论:HFrD 会导致 IHL 积累和糖尿病的发生,而 VDS 和 HIIT 以及它们的联合作用可以预防 HFrD 的后果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amelioration of fructose-induced hepatic lipid accumulation by vitamin D3 supplementation and high-intensity interval training in male Sprague‒Dawley rats.

Background: Intrahepatic lipid accumulation (IHL), a hallmark of metabolic disorders, is closely associated with de novo lipogenesis (DNL). Notably, fructose feeding increased the DNL. Lifestyle modifications resulting from dietary changes and increased physical activity/exercise can decrease the IHL content. We examined the effects of vitamin D3 supplementation (VDS), high-intensity interval training (HIIT), and their combination on the transcription factors and enzymes of the DNL pathway in male Sprague‒Dawley rats fed a high-fructose diet (HFrD).

Methods: Forty male rats were assigned to 5 groups (n = 8): CS (the control group had a standard diet); CF (the control group had HFrD (10% (w/v) fructose solution in tap water)); and FT (HFrD + HIIT: 10 bouts of 4 min of high-intensity running, corresponding to 85-90% of the maximal speed with 2 min active rest periods of 50% maximal speed, 5 days per week); FD (HFrD + intervention of intraperitoneal injection of 10000 IU/kg/week VDS); FTD (HFrD + HIIT + VDS) that were maintained for 12 weeks. ELISA, the GOD-POD assay, folch, western blotting, and oil red O staining were used to determine insulin, fasting blood glucose (FBG), hepatic triglyceride (TG) and cholesterol levels; SREBP1c, ChREBP-β, ACC1, FASN, p-ACC1, AMPK, p-AMPK, and PKA protein expression; and IHL content, respectively.

Results: Both HIIT and VDS led to significant increases in the levels of PKA, AMPK, p-AMPK, and p-ACC1, as well as significant decreases in the levels of SREBP1c, ChREBP-β, ACC1, FASN, insulin, FBG, liver TG, liver cholesterol, and IHL. HIIT exhibited superior efficacy over VDS in reducing ChREBP-β, ACC1, insulin, FBG, liver TG and cholesterol, as well as increasing p-ACC1 and PKA. Notably, the combined intervention of HIIT and VDS yielded the most substantial improvements across all the parameters.

Conclusions: HFrD causes IHL accumulation and the onset of diabetes, whereas VDS and HIIT, along with their combined effects, prevent the consequences of HFrD.

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来源期刊
Lipids in Health and Disease
Lipids in Health and Disease 生物-生化与分子生物学
CiteScore
7.70
自引率
2.20%
发文量
122
审稿时长
3-8 weeks
期刊介绍: Lipids in Health and Disease is an open access, peer-reviewed, journal that publishes articles on all aspects of lipids: their biochemistry, pharmacology, toxicology, role in health and disease, and the synthesis of new lipid compounds. Lipids in Health and Disease is aimed at all scientists, health professionals and physicians interested in the area of lipids. Lipids are defined here in their broadest sense, to include: cholesterol, essential fatty acids, saturated fatty acids, phospholipids, inositol lipids, second messenger lipids, enzymes and synthetic machinery that is involved in the metabolism of various lipids in the cells and tissues, and also various aspects of lipid transport, etc. In addition, the journal also publishes research that investigates and defines the role of lipids in various physiological processes, pathology and disease. In particular, the journal aims to bridge the gap between the bench and the clinic by publishing articles that are particularly relevant to human diseases and the role of lipids in the management of various diseases.
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