古 DNA 分析中应用的不同研究技术的成本效益及其他考虑因素。

IF 1.4 Q3 ANATOMY & MORPHOLOGY
Jong Ha Hong, Hisashi Fujita, Jaehyup Kim, Dong Hoon Shin
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引用次数: 0

摘要

古 DNA(aDNA)分析自 20 世纪 80 年代首次出现以来发展迅速,几乎成为人类学和考古学中不可或缺的工具。早期的 aDNA 研究以聚合酶链式反应(PCR)技术为基础,但遗憾的是,这种技术经常出现现代 DNA 污染和其他真实性问题。新一代测序(NGS)技术等先进技术的应用很快克服了这些技术障碍。然而,由于 NGS 需要资金、时间,在大型项目中还需要人力,一些被认为无足轻重的古代样本的基因分析通常会被推迟,最糟糕的情况是完全被忽视。我们承认,作为 aDNA 分析中的一种诊断工具,PCR 的准确性不如 NGS,而且更容易受到现代 DNA 污染的影响;但它也有一些优点,如简单、省时、更易于解释等。因此,应重新考虑 PCR 在 aDNA 分析中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cost-effectiveness and other considerations for different research techniques applied in ancient DNA analysis.

Ancient DNA (aDNA) analysis has developed rapidly since it first emerged in the 1980s, becoming an almost indispensable tool in anthropological and archaeological sciences. Earlier aDNA study was based on the polymerase chain reaction (PCR) technique, with which, unfortunately, modern DNA contamination and other authenticity issues were often incurred. These technical hurdles were soon overcome by application of advancements in the forms of the next generation sequencing (NGS) technique and others. However, since NGS requires money, time, and, in the case of large projects, manpower as well, genetic analysis of some ancient samples considered to be insignificant is commonly delayed or, in the worst cases, neglected entirely. We acknowledge that as a diagnostic tool in aDNA analysis, PCR is less accurate than NGS and more easily affected by modern DNA contamination; but it also has advantages, such as simplicity, time-saving, and greater ease of interpretation, among others. The role of PCR in aDNA analysis, then, should be reconsidered.

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来源期刊
Anatomy & Cell Biology
Anatomy & Cell Biology ANATOMY & MORPHOLOGY-
CiteScore
1.80
自引率
9.10%
发文量
75
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