体外诱导的冷记忆不能促进 Araucaria angustifolia 胚胎培养物的发育。

IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Anais da Academia Brasileira de Ciencias Pub Date : 2024-11-04 eCollection Date: 2024-01-01 DOI:10.1590/0001-3765202420240574
Gabriel Antônio P DE Matos, Evelyn L Dos Santos, Yohan Fritsche, Thiago S Ornellas, Neusa Steiner, Valdir Marcos Stefenon
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引用次数: 0

摘要

本研究旨在研究将Araucaria angustifolia的体细胞胚胎置于体外低温环境中如何促进体细胞胚胎的发生。体细胞胚胎由未成熟的合子胚胎诱导而来,生长 120 天,然后在 4.5°C 的温度下培养 0、2、4、8 或 16 天。冷处理后,将胚胎发生培养物放回 25±2°C 20 天,用光学显微镜和细胞化学方法进行检查。结果发现,处于 PEM III 阶段的胚胎发生培养物没有进一步的发育,而且在较长时间的冷处理中,胚胎发生体的生长受到了负面影响。要克服A. angustifolia体细胞胚胎发生方案中的这些挑战,需要进一步对驱动子代胚胎发育的表观遗传事件进行综合研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro induced cold memory fails to enhance embryogenic cultures development in Araucaria angustifolia.

This research aimed to study how exposing somatic embryos of Araucaria angustifolia to cold temperatures in vitro could enhance somatic embryogenesis. The somatic embryos were induced from immature zygotic embryos, grown for 120 days, and then subjected to 4.5°C for 0, 2, 4, 8, or 16 days. After the cold treatment, the embryogenic cultures were returned to 25±2°C for 20 days and examined using light microscopy and cytochemistry. It was found that the embryogenic cultures at the PEM III stage did not show further development, and the growth of the embryogenic masses was negatively affected in the longer-duration cold treatment. Overcoming these challenges in the somatic embryogenesis protocol for A. angustifolia requires further integrative investigations on epigenetic events driving the zygotic embryo development.

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来源期刊
Anais da Academia Brasileira de Ciencias
Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊
CiteScore
2.20
自引率
0.00%
发文量
347
审稿时长
1 months
期刊介绍: The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.
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