利用茄皮合成的银纳米粒子的兼容性和抗菌活性。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Esraa Ali, Samah H Abu-Hussien, Esraa Hesham, Shimaa Ahmed, Habiba Mostafa, Ahmed Gamal, Salwa M El-Sayed, Bahaa Hemdan, Ashraf Bakry, Naglaa M Ebeed, Hesham Elhariry, Ahmed Galal, Basma T Abd-Elhalim
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引用次数: 0

摘要

纳米粒子因其特殊的理化性质,已成为化学防治剂的新替代品,受到全世界的关注。目前的研究重点是利用枸杞果皮以环境友好的方式合成银纳米粒子(AgNPs)。除了研究Le-AgNPs的内在细胞毒性效力有助于其抗菌和抗真菌活性外,还研究了纳米粒子对其形态行为完整性的影响。将 L. esculentum(Le)提取物与硝酸银(AgNO3)溶液混合后,其颜色由淡粉色变为淡棕色,这表明 L. esculentum(Le)银纳米粒子(Le-AgNPs)的主动生物合成。利用紫外可见光谱、动态光散射(DLS)、傅立叶变换红外光谱、高分辨率透射电子显微镜(HR-TEM)和 X 射线衍射技术对生物合成的 Le-AgNPs 进行了表征。紫外可见光谱结果表明,SPR 为 2.5 时,表面等离子体共振波长为 310 纳米。DLS 结果显示,颗粒大小为 186 nm,多分散指数为 0.573。傅立叶变换红外光谱显示存在羧基、羟基、酚基和酰胺官能团。HR-TEM 分析显示,Le-AgNPs 呈准球形晶体颗粒。Le-AgNPs 的 zeta 电位为负值 - 68.44 mV,表明其具有很高的稳定性。枯草芽孢杆菌(Bacillus subtilis ATCC 6633)和大肠杆菌(Escherichia coli ATCC 8739)是最易受 Le-AgNPs 抑制的病原体,抑制区直径(IZD)分别为 4.0 厘米和 0.92 厘米。然而,单核细胞增生李斯特菌 NC 013768 和宋内志贺氏菌 DSM 5570 是最具抵抗力的病原体,其抑制区直径分别为 0.92 厘米和 0.90 厘米。Le-AgNPs 对病原真菌具有良好的抑制潜力,对 Alternaria solani ATCC 62102 和 Candida albicans DSM 1386 的 IZD 分别为 3.0 厘米和 0.92 厘米。对人体结肠 NCM460D 正常细胞的半数最大抑制浓度(IC50)为 200.53 μg/ml,观察到了细胞毒性效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Compatibility and antimicrobial activity of silver nanoparticles synthesized using Lycopersicon esculentum peels.

Nanoparticles have gained worldwide attention as a new alternative to chemical control agents due to their special physiochemical properties. The current study focused on the environmentally friendly synthesis of silver nanoparticles (AgNPs) using Lycopersicon esculentum peel. In addition to studying the intrinsic cytotoxic effectiveness of Le-AgNPs contribute to their antibacterial, and antifungal activities and the effect of nanoparticles on the integrity of their morphological behavior. The initiative biosynthesis of L. esculentum silver nanoparticles (Le-AgNPs) was indicated by the color change of L. esculentum (Le) extract mixed with silver nitrate (AgNO3) solution from faint pink to faint brown. UV-visible spectroscopy, Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction techniques were used to characterize biosynthesized Le-AgNPs. Results of UV-visible spectroscopy recorded surface plasmon resonance at 310 nm for SPR of 2.5. The DLS results showed particles of 186 nm with a polydispersity index of 0.573. The FTIR spectrum indicated the existence of carboxyl, hydroxyl, phenolic, and amide functional groups. The HR-TEM analysis revealed quasi-spherical crystal particles of Le-AgNPs. Le-AgNPs had a negative zeta potential of - 68.44 mV, indicating high stability. Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 8739 were the most susceptible pathogens to Le-AgNPs inhibition, with inhibition zone diameters (IZDs) of 4.0 and 0.92 cm, respectively. However, Listeria monocytogenes NC 013768 and Shigella sonnei DSM 5570 were the most resistant pathogens, with IZDs of 0.92 and 0.90 cm, respectively. Le-AgNPs demonstrated good inhibitory potential against pathogenic fungi, with IZDs of 3.0 and 0.92 cm against Alternaria solani ATCC 62102 and Candida albicans DSM 1386, respectively. The cytotoxicity effect was observed at a half-maximal inhibitory concentration (IC50) of 200.53 μg/ml on human colon NCM460D normal cells.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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