Sunbum Kwon, Vasily Morozov, Lingfei Wang, Pradeep K Mandal, Stéphane Chaignepain, Céline Douat, Ivan Huc
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引用次数: 0
摘要
除了在其表面显示各种极性和疏水侧链外,我们还设计了一种生物素化的螺旋芳香族寡酰胺折叠聚合物,其大小相当于 24 聚肽。该产品在固相上合成,其 P-和 M-螺旋构象在手性固定相上通过高效液相色谱进行分离,并通过 X 射线晶体学阐明了非生物素化类似物的固态结构。以折叠酶为诱饵从酵母细胞裂解物中进行拉取实验,然后进行蛋白质组分析,发现了潜在的蛋白质结合伙伴。其中三种蛋白质被重组表达。生物层干涉测量法显示了亚摩尔级的结合力,这表明在没有设计考虑的情况下,特定的折叠聚合体对某些蛋白质具有亲和力。然而,这三种情况下的结合选择性都很低,因为 P 型和 M 型折叠体与蛋白质的结合亲和力相似。
Interrogating the potential of helical aromatic foldamers for protein recognition.
A biotinylated helical aromatic oligoamide foldamer equivalent in size to a 24mer peptide was designed without any prejudice other than to display various polar and hydrophobic side chains at its surface. It was synthesized on solid phase, its P- and M-helical conformers were separated by HPLC on a chiral stationary phase, and the solid state structure of a non-biotinylated analogue was elucidated by X-ray crystallography. Pull-down experiments from a yeast cell lysate using the foldamer as a bait followed by proteomic analysis revealed potential protein binding partners. Three of these proteins were recombinantly expressed. Biolayer interferometry showed submicromolar binding demonstrating the potential of a given foldamer to have affinity for certain proteins in the absence of design considerations. Yet, binding selectivity was low in all three cases since both P- and M-conformers bound to the proteins with similar affinities.