通过碳化聚合物点纳米传感器利用原位双光子荧光光谱精确揭示细胞内脂滴微环境

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-05 DOI:10.1016/j.snb.2024.136900

Zitong Yu , Xiumian Cao , Zepeng Huo , Weiqing Xu , Shuping Xu

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引用次数: 0

摘要

脂滴（LDs）是维持脂质平衡和支持生物交流的重要动态细胞器。脂滴的极性至关重要，因为它们的异质性与病理生理发展密切相关。为了研究这些变化，我们开发了一种极性特异性双光子荧光（TPF）平台，利用碳化聚合物点（CPD）探针实时、精确地监测细胞内 LDs 的极性微环境。该平台可对单个 LD 进行准确的原位极性评估。利用这一平台，我们确定了癌细胞和非癌细胞之间 LD 极性的差异，以及铁质酸化和脂肪变性等病理生理过程中 LD 极性的变化。与 TPF 光谱相关的 CPD 探针的开发标志着在了解 LD 在脂质相关疾病中的作用方面取得了重大进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

In situ two-photon fluorescence spectroscopy to precisely reveal intracellular lipid droplet microenvironment via a carbonized polymer dot nanosensor

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In situ two-photon fluorescence spectroscopy to precisely reveal intracellular lipid droplet microenvironment via a carbonized polymer dot nanosensor

Lipid droplets (LDs) are vital dynamic cellular organelles that maintain lipid equilibrium and support biological communication. The polarity of LDs is critical, as their heterogeneity is closely related to pathophysiological developments. To investigate these changes, we developed a polarity-specific two-photon fluorescence (TPF) platform for real-time and precise monitoring of the polarity microenvironment of intracellular LDs with a carbonized polymer dots (CPD) probe. This platform enables the accurate and in situ polarity assessment of a single LD. Using this platform, we identified differences in LD polarity between cancerous and non-cancerous cells, as well as variations in LD polarity during pathophysiological events such as ferroptosis and steatosis. The development of the CPD probe associated with TPF spectroscopy marks a significant advancement in understanding the role of LDs in lipid-associated diseases.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.