提高免疫组化效率的机械微振动装置

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Weifeng Zhang, Jirui Li, Fengshan Xie, Liting Zeng, Liangli Hong, Penghao Li, Xiaomiao Yan, Jingliang Xu, Meina Du, Jiongzhi Hong, Dingrong Yi, Jiahao Xie, Jiang Gu
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引用次数: 0

摘要

免疫组化(IHC)是一种广泛应用于病理诊断和生物医学研究的技术,但仍需缩短操作流程并降低抗体成本。本研究旨在评估一种新型 IHC 技术,该技术结合了机械微振动(MMV)技术,可加快操作流程、减少抗体消耗并提高染色质量。MMV是使用硬币振动电机产生的,该电机连接到装有连续组织切片的玻璃载玻片上。针对不同抗原的多种抗体被用于对癌症和正常组织进行染色,包括使用和不使用微振动。测试了各种参数,包括孵育时间、温度和抗体稀释度。结果表明,这种新方法可以在更短的时间内取得相当或更好的效果,使用的抗体比对照组少 10 倍以上。MMV 提高了特异性染色质量,可产生更强、更清晰的阳性反应。多中心双盲评估和定量图像分析验证了这一点。此外,还对可能的机制进行了研究。MMV 可缩短免疫组化染色时间,减少抗体用量,提高染色特异性,这可能是通过加速抗体的移动和扩散实现的。这些改进节省了时间和成本,为病理诊断和生物医学研究提供了临床和经济价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency

Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency

Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology and biomedical research, but there is still a need to shorten the operation process and reduce the cost of antibodies. This study aims to assess a novel IHC technique that incorporates mechanical microvibration (MMV) to expedite the process, reduce antibody consumption, and enhance staining quality. MMV was generated using coin vibration motors attached to glass slides mounted with consecutive tissue sections. Multiple antibodies targeting various antigens were used to stain cancerous and normal tissues, with and without microvibration. Various parameters were tested, including incubation durations, temperatures, and antibody dilutions. The novel method showed the potential to achieve comparable or superior outcomes in significantly less time, utilizing over 10 times less antibody than controls. MMV improved specific staining quality, yielding stronger, and better-defined positive reactions. This was validated through a multicenter double-blind assessment and quantitative image analysis. The possible mechanisms were also investigated. MMV shortens immunohistochemical staining duration, reduces antibody usage, and enhances staining specificity, likely by accelerating antibody movement and diffusion. These improvements translate to time and cost savings, offering clinical and financial value for diagnostic pathology and biomedical research.

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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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