最大限度地提高层状双氢氧化物作为缓释磷肥的效率：关于植物生长促进根瘤菌影响的研究

IF 5.3 2区地球科学 Q2 CHEMISTRY, PHYSICAL

Applied Clay Science Pub Date : 2024-11-04 DOI:10.1016/j.clay.2024.107620

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引用次数: 0

摘要

层状双氢氧化物（LDH）因其在农业中的潜在应用而备受关注，它是植物必需营养元素的缓释源。本研究旨在探讨磷酸盐插层的 Mg-Al-LDH 材料（M2+/M3+比例为 2:1 和 3:1）在含有或不含有两种植物生长促进根瘤菌（PGPR）菌株的情况下，对玉米生长以及磷（P）、镁（Mg）和锰（Mn）吸收的影响。合成了 LDH 材料，并通过 X 射线衍射（XRD）图谱、傅立叶变换红外光谱（FT-IR）和元素分析评估了它们的特性。在体外实验中，对五种 PGPR 菌株的 P 溶解能力进行了评估，并挑选出两种最有效的菌株（炭疽芽孢杆菌：B1 & ；假单胞菌：B2）用于随后的盆栽试验（温室）。在盆栽试验中，这两种细菌菌株的功效与玉米植株从特定数量的 Mg-Al-LDH-P（2:1）和 Mg-Al-LDH-P（3:1）材料中获取钾的情况有关，这两种材料分别添加在 500 克土壤中，提供的钾浓度分别为 0、50 和 100 毫克/千克。此外，还加入了三重过磷酸钙（TSP）作为快速释放钾源（50 毫克 P kg-1）。无论使用哪种 LDH，施用 LDHs 都能显著增加玉米植株的芽和根的生物量；受 LDH 类型和 P 含量的影响，LDH 的效果与 TSP 相似，甚至更高。与未处理的对照组相比，施用 LDHs 和 TSP 可提高玉米植株嫩枝的 P 浓度。在没有任何 P 处理的情况下，接种这两种细菌菌株会增加玉米植株的芽 P 浓度。此外，在 LDH 2:1 （100 毫克 P kg-1）和 TSP 处理中，两种细菌菌株都能提高植株的芽 P 浓度，这表明 PGPR 与 LDH 2:1 和 TSP 之间存在协同作用（而 PGPR 与其他 LDH 处理之间没有协同作用）。在不施用任何 LDH/TSP 的情况下，与未接种对照相比，只有 B1 菌株能增加植物的芽和根的生物量。就嫩枝生物量而言，与单独施用 PGPR 和 LDH 相比，任何 PGPR-LDH 组合都未观察到协同效应，这可能是由于植物的生长期较短（40 天）。总之，这项研究表明，LDH（2,1）和 LDH（3,1）可以有效地作为缺钾条件下促进植物生长的钾源。此外，研究结果还强调了 PGPR 菌株从含 P 的 LDH 化合物中释放 P 的潜力，这可能会根据 LDH 中金属阳离子的摩尔比而有所不同。

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Maximizing the efficiency of layered double hydroxides as a slow-release phosphate fertilizer: A study on the impact of plant growth-promoting rhizobacteria

Layered double hydroxides (LDH) have gained considerable attention for their potential application in agriculture, serving as a slow-release source of essential nutrients for plants. This study aimed to investigate the effects of Mg-Al-LDH materials (with M²⁺/M³⁺ ratios of 2:1 and 3:1) intercalated with phosphate, both with and without the presence of two plant growth-promoting rhizobacteria (PGPR) strains, on maize growth and the uptake of phosphorus (P), magnesium (Mg), and manganese (Mn). The LDH materials were synthesized, and their properties were assessed by X-ray diffraction (XRD) patterns, Fourier-transform infrared (FT-IR) spectra, and elemental analysis. In an in vitro experiment, the P solubilization capacity of five PGPR strains was evaluated, and the two most effective strains (Bacillus anthracis: B1 & Pseudomonas sp.: B2) were selected for the subsequent pot trials (greenhouse). In the pot experiment, the effectiveness of these two bacterial strains was evaluated in relation to the availability of P for maize plants from specific quantities of Mg-Al-LDH-P (2:1) and Mg-Al-LDH-P (3:1) materials amended in 500 g of soil, providing P concentrations of 0, 50, and 100 mg kg⁻¹. A treatment of triple superphosphate (TSP) was also included as a fast-release P source (50 mg P kg⁻¹). Irrespective of the type of LDH used, the application of LDHs significantly increased the shoot and root biomass of maize plants; the LDH effect was similar or even higher than that of the TSP, driven by the LDH type and P content. The application of LDHs and TSP resulted in higher shoot P concentration in maize plants compared to the untreated control. Inoculation with both bacterial strains increased the shoot P concentration of maize plants in the absence of any P treatments. Moreover, both bacterial strains increased the shoot P concentration of plants in LDH 2:1 (100 mg P kg⁻¹) and TSP treatments, indicating synergistic interactions between PGPR and LDH 2:1 and TSP (while no synergism was found between PGPR and other LDH treatments). Without any LDH/TSP application, only the B1 strain increased the shoot and root biomass of plants compared to the non-inoculated control. In terms of shoot biomass, no synergistic effects were observed in any PGPR-LDH combinations compared to the individual application of PGPR and LDH, which could possibly be due to the short growth period (40 days) of plants. Overall, this study suggests that LDH (2,1) and LDH (3,1) can effectively serve as a P source for enhancing plant growth under P deficiency conditions. Furthermore, the results underscore the potential of PGPR strains to release P from P-containing LDH compounds, which could vary based on the molar ratios of metal cations in LDHs.

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来源期刊

Applied Clay Science 地学-矿物学

CiteScore

10.30

自引率

10.70%

发文量

289

审稿时长

39 days

期刊介绍： Applied Clay Science aims to be an international journal attracting high quality scientific papers on clays and clay minerals, including research papers, reviews, and technical notes. The journal covers typical subjects of Fundamental and Applied Clay Science such as: • Synthesis and purification • Structural, crystallographic and mineralogical properties of clays and clay minerals • Thermal properties of clays and clay minerals • Physico-chemical properties including i) surface and interface properties; ii) thermodynamic properties; iii) mechanical properties • Interaction with water, with polar and apolar molecules • Colloidal properties and rheology • Adsorption, Intercalation, Ionic exchange • Genesis and deposits of clay minerals • Geology and geochemistry of clays • Modification of clays and clay minerals properties by thermal and physical treatments • Modification by chemical treatments with organic and inorganic molecules(organoclays, pillared clays) • Modification by biological microorganisms. etc...