利用质谱法识别水生病毒编码的新型蛋白质

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2024-10-31 DOI:10.1016/j.virol.2024.110281

Fei Yu, Siyang Song, Jiehua Xu, Kai Hao, Yu Wang, Zhe Zhao

{"title":"利用质谱法识别水生病毒编码的新型蛋白质","authors":"Fei Yu, Siyang Song, Jiehua Xu, Kai Hao, Yu Wang, Zhe Zhao","doi":"10.1016/j.virol.2024.110281","DOIUrl":null,"url":null,"abstract":"<div><h3>Aquareovirus</h3><div>a genus of within the family <em>Spinareoviridae</em>, order <em>Reovirales</em>, infects aquatic animals. Their genomes comprise 11 segments of double-stranded RNA, which function directly as mRNAs upon release into the cytoplasm of infected cells. Here, liquid chromatography-tandem mass spectrometry was employed to annotate small coding ORFs in the Aquareovirus-C genome. Its plus-strand RNA of segment 8 (S8) contains a novel protein-coding frame (NS15), and S5 seems to has an additional reading frame (NS18) with a putative non-AUG initiation codon. Among them, NS15 polypeptide has been proved by immunoblotting assay. Remarkably, the S4 and S11 minus-strand mRNAs may encode polypeptides, suggesting ambisense polarity of the two segmented RNAs. And the newly discovered NS12 ORF in 2019, from viral tricistronic S7 mRNA, was also confirmed by this mass-spectrometry data. Taken together, these identified new ORFs reveal the genome-coding complexity of Aquareovirus-C.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"601 ","pages":"Article 110281"},"PeriodicalIF":2.8000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Recognition of novel proteins encoded by an aquareovirus using mass spectrometry\",\"authors\":\"Fei Yu, Siyang Song, Jiehua Xu, Kai Hao, Yu Wang, Zhe Zhao\",\"doi\":\"10.1016/j.virol.2024.110281\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aquareovirus</h3><div>a genus of within the family <em>Spinareoviridae</em>, order <em>Reovirales</em>, infects aquatic animals. Their genomes comprise 11 segments of double-stranded RNA, which function directly as mRNAs upon release into the cytoplasm of infected cells. Here, liquid chromatography-tandem mass spectrometry was employed to annotate small coding ORFs in the Aquareovirus-C genome. Its plus-strand RNA of segment 8 (S8) contains a novel protein-coding frame (NS15), and S5 seems to has an additional reading frame (NS18) with a putative non-AUG initiation codon. Among them, NS15 polypeptide has been proved by immunoblotting assay. Remarkably, the S4 and S11 minus-strand mRNAs may encode polypeptides, suggesting ambisense polarity of the two segmented RNAs. And the newly discovered NS12 ORF in 2019, from viral tricistronic S7 mRNA, was also confirmed by this mass-spectrometry data. Taken together, these identified new ORFs reveal the genome-coding complexity of Aquareovirus-C.</div></div>\",\"PeriodicalId\":23666,\"journal\":{\"name\":\"Virology\",\"volume\":\"601 \",\"pages\":\"Article 110281\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0042682224003052\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0042682224003052","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

摘要

水生病毒属 Spinareoviridae 科 Reovirales 目，感染水生动物。它们的基因组由 11 段双链 RNA 组成，在释放到受感染细胞的细胞质中时直接作为 mRNA 起作用。本文采用液相色谱-串联质谱法注释了水生病毒-C基因组中的小编码ORF。其第8段的加链RNA（S8）含有一个新的蛋白质编码框（NS15），S5似乎有一个额外的阅读框（NS18），并有一个假定的非AUG起始密码子。其中，NS15 多肽已通过免疫印迹试验得到证实。值得注意的是，S4 和 S11 负链 mRNA 可能编码多肽，这表明这两种分段 RNA 具有歧义极性。质谱数据还证实了 2019 年新发现的 NS12 ORF，该 ORF 来自病毒三链式 S7 mRNA。综上所述，这些新发现的ORF揭示了水生病毒-C基因组编码的复杂性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Recognition of novel proteins encoded by an aquareovirus using mass spectrometry

Aquareovirus

a genus of within the family Spinareoviridae, order Reovirales, infects aquatic animals. Their genomes comprise 11 segments of double-stranded RNA, which function directly as mRNAs upon release into the cytoplasm of infected cells. Here, liquid chromatography-tandem mass spectrometry was employed to annotate small coding ORFs in the Aquareovirus-C genome. Its plus-strand RNA of segment 8 (S8) contains a novel protein-coding frame (NS15), and S5 seems to has an additional reading frame (NS18) with a putative non-AUG initiation codon. Among them, NS15 polypeptide has been proved by immunoblotting assay. Remarkably, the S4 and S11 minus-strand mRNAs may encode polypeptides, suggesting ambisense polarity of the two segmented RNAs. And the newly discovered NS12 ORF in 2019, from viral tricistronic S7 mRNA, was also confirmed by this mass-spectrometry data. Taken together, these identified new ORFs reveal the genome-coding complexity of Aquareovirus-C.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.