Marcos Jessé Abrahão Silva, Thiago Pinto Brasil, Caroliny Soares Silva, Cristiane Cunha Frota, Daniele Melo Sardinha, Luiza Raquel Tapajós Figueira, Keitty Anne Silva Neves, Everaldina Cordeiro Dos Santos, Karla Valéria Batista Lima, Nédia de Castilhos Ghisi, Luana Nepomuceno Gondim Costa Lima
{"title":"通过使用 16S rRNA 基因进行 PCR 检测麻风病临床病谱检出率的比较分析：科学计量学和荟萃分析。","authors":"Marcos Jessé Abrahão Silva, Thiago Pinto Brasil, Caroliny Soares Silva, Cristiane Cunha Frota, Daniele Melo Sardinha, Luiza Raquel Tapajós Figueira, Keitty Anne Silva Neves, Everaldina Cordeiro Dos Santos, Karla Valéria Batista Lima, Nédia de Castilhos Ghisi, Luana Nepomuceno Gondim Costa Lima","doi":"10.3389/fmicb.2024.1497319","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Leprosy is a chronic and disabling infectious disease caused by <i>Mycobacterium leprae</i>. It has a wide clinical spectrum and is operationally classified into paucibacillary (PB) and multibacillary (MB) cases. There is evidence that the <i>16S rRNA</i> gene can be used in Polymerase Chain Reaction (PCR) for complementary detection with high sensitivity and specificity. However, there is no literature convention on its diagnostic correspondence regarding the particular operational classification of the disease. This study aimed to correlate, through a meta-analysis, the detection rate of leprosy between the PCR method with the <i>16S rRNA</i> gene in the clinical forms PB and MB in relation to confirmed cases.</p><p><strong>Methods: </strong>This is a systematic review and meta-analysis study conducted according to the PRISMA 2020 guidelines, using the search descriptors with \"AND\": \"Leprosy\"; \"Polymerase Chain Reaction\"; \"<i>16S rRNA</i>\" in the PUBMED, SciELO, LILACS, and Science Direct databases. The search was limited to original observational articles in Portuguese, English, or Spanish, with no defined time frame. The methodological quality assessment of the selected articles was performed using the JBI checklists. A scientometric approach to the article using used the VOS Viewer and Scimago Graphica software. The meta-analysis was conducted using Comprehensive Meta-Analyses software, under Pearson's Correlation effect test and fixed effect model and subgroup analysis concerning the type of sample analyzed.</p><p><strong>Results: </strong>The study was significant from the perspective of the paucibacillary group (Clinical biopsy: -0.45 [95% CI= -0.63 - -0.22], <i>p</i> < 0.001/ Slit smear skin: -0.52 [95% CI= -0.65 - -0.36], <i>p</i> < 0.001 / Overall: -0.50 [95% CI= -0.61 - -0.37], <i>p</i> < 0.001). The PCR diagnostic method for the16S rRNAgene ofM. lepraehas low viability and diagnostic sensitivity in both clinical biopsy samples and leprosy skin smears.</p><p><strong>Conclusion: </strong>This implies little validation of it as a PCR target gene for diagnosing the disease, highlighting limitations in the actual technique.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/prospero/, identifier CRD42024588790.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532149/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using the <i>16S rRNA</i> gene: a scientometrics and meta-analysis.\",\"authors\":\"Marcos Jessé Abrahão Silva, Thiago Pinto Brasil, Caroliny Soares Silva, Cristiane Cunha Frota, Daniele Melo Sardinha, Luiza Raquel Tapajós Figueira, Keitty Anne Silva Neves, Everaldina Cordeiro Dos Santos, Karla Valéria Batista Lima, Nédia de Castilhos Ghisi, Luana Nepomuceno Gondim Costa Lima\",\"doi\":\"10.3389/fmicb.2024.1497319\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Leprosy is a chronic and disabling infectious disease caused by <i>Mycobacterium leprae</i>. It has a wide clinical spectrum and is operationally classified into paucibacillary (PB) and multibacillary (MB) cases. There is evidence that the <i>16S rRNA</i> gene can be used in Polymerase Chain Reaction (PCR) for complementary detection with high sensitivity and specificity. However, there is no literature convention on its diagnostic correspondence regarding the particular operational classification of the disease. This study aimed to correlate, through a meta-analysis, the detection rate of leprosy between the PCR method with the <i>16S rRNA</i> gene in the clinical forms PB and MB in relation to confirmed cases.</p><p><strong>Methods: </strong>This is a systematic review and meta-analysis study conducted according to the PRISMA 2020 guidelines, using the search descriptors with \\\"AND\\\": \\\"Leprosy\\\"; \\\"Polymerase Chain Reaction\\\"; \\\"<i>16S rRNA</i>\\\" in the PUBMED, SciELO, LILACS, and Science Direct databases. The search was limited to original observational articles in Portuguese, English, or Spanish, with no defined time frame. The methodological quality assessment of the selected articles was performed using the JBI checklists. A scientometric approach to the article using used the VOS Viewer and Scimago Graphica software. The meta-analysis was conducted using Comprehensive Meta-Analyses software, under Pearson's Correlation effect test and fixed effect model and subgroup analysis concerning the type of sample analyzed.</p><p><strong>Results: </strong>The study was significant from the perspective of the paucibacillary group (Clinical biopsy: -0.45 [95% CI= -0.63 - -0.22], <i>p</i> < 0.001/ Slit smear skin: -0.52 [95% CI= -0.65 - -0.36], <i>p</i> < 0.001 / Overall: -0.50 [95% CI= -0.61 - -0.37], <i>p</i> < 0.001). The PCR diagnostic method for the16S rRNAgene ofM. lepraehas low viability and diagnostic sensitivity in both clinical biopsy samples and leprosy skin smears.</p><p><strong>Conclusion: </strong>This implies little validation of it as a PCR target gene for diagnosing the disease, highlighting limitations in the actual technique.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/prospero/, identifier CRD42024588790.</p>\",\"PeriodicalId\":12466,\"journal\":{\"name\":\"Frontiers in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-10-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532149/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fmicb.2024.1497319\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1497319","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：麻风病是一种由麻风分枝杆菌引起的慢性致残性传染病：麻风病是由麻风分枝杆菌引起的一种慢性致残性传染病。麻风病的临床表现范围很广，在操作上可分为贫脓疱型（PB）和多脓疱型（MB）病例。有证据表明，16S rRNA 基因可用于聚合酶链式反应（PCR）的互补检测，具有很高的灵敏度和特异性。然而，目前还没有关于该疾病特定操作分类的诊断对应关系的文献惯例。本研究旨在通过一项荟萃分析，对PCR方法与16S rRNA基因在临床形式为PB和MB的确诊病例中的麻风病检出率进行关联分析：这是一项根据 PRISMA 2020 指南进行的系统综述和荟萃分析研究，使用了带 "AND "的搜索描述符："麻风病"；"聚合酶链式反应"；"16S rRNA"。搜索仅限于葡萄牙文、英文或西班牙文的原始观察性文章，没有限定时间范围。对所选文章的方法学质量评估采用了 JBI 核对表。文章的科学计量学方法使用了 VOS Viewer 和 Scimago Graphica 软件。使用 Comprehensive Meta-Analyses 软件进行了荟萃分析，采用了皮尔逊相关效应检验和固定效应模型，并对分析样本的类型进行了分组分析：从贫瘠组的角度来看，该研究具有重要意义（临床活检：-0.45 [95% CI= -0.63 - -0.22]，p p p 结论：从贫瘠组的角度来看，该研究具有重要意义（临床活检：-0.45 [95% CI= -0.63 - -0.22]，p p p 结论）：这意味着将其作为诊断疾病的 PCR 目标基因几乎没有得到验证，凸显了实际技术的局限性。系统综述注册：https://www.crd.york.ac.uk/prospero/，标识符为 CRD42024588790。
Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using the 16S rRNA gene: a scientometrics and meta-analysis.
Background: Leprosy is a chronic and disabling infectious disease caused by Mycobacterium leprae. It has a wide clinical spectrum and is operationally classified into paucibacillary (PB) and multibacillary (MB) cases. There is evidence that the 16S rRNA gene can be used in Polymerase Chain Reaction (PCR) for complementary detection with high sensitivity and specificity. However, there is no literature convention on its diagnostic correspondence regarding the particular operational classification of the disease. This study aimed to correlate, through a meta-analysis, the detection rate of leprosy between the PCR method with the 16S rRNA gene in the clinical forms PB and MB in relation to confirmed cases.
Methods: This is a systematic review and meta-analysis study conducted according to the PRISMA 2020 guidelines, using the search descriptors with "AND": "Leprosy"; "Polymerase Chain Reaction"; "16S rRNA" in the PUBMED, SciELO, LILACS, and Science Direct databases. The search was limited to original observational articles in Portuguese, English, or Spanish, with no defined time frame. The methodological quality assessment of the selected articles was performed using the JBI checklists. A scientometric approach to the article using used the VOS Viewer and Scimago Graphica software. The meta-analysis was conducted using Comprehensive Meta-Analyses software, under Pearson's Correlation effect test and fixed effect model and subgroup analysis concerning the type of sample analyzed.
Results: The study was significant from the perspective of the paucibacillary group (Clinical biopsy: -0.45 [95% CI= -0.63 - -0.22], p < 0.001/ Slit smear skin: -0.52 [95% CI= -0.65 - -0.36], p < 0.001 / Overall: -0.50 [95% CI= -0.61 - -0.37], p < 0.001). The PCR diagnostic method for the16S rRNAgene ofM. lepraehas low viability and diagnostic sensitivity in both clinical biopsy samples and leprosy skin smears.
Conclusion: This implies little validation of it as a PCR target gene for diagnosing the disease, highlighting limitations in the actual technique.
期刊介绍：
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
