Gilberto Manzo-Sánchez, Luis Carlos Salazar-Licea, Marco Tulio Buenrostro-Nava, Carlos L. Leopardi-Verde, Luciano Martínez-Bolaños, Ignacio Islas-Flores, Mario Orozco-Santos, Blondy Canto-Canché
{"title":"墨西哥香蕉病原体 Pseudocercospora fijiensis 的 QoI 抗性分离物鉴定","authors":"Gilberto Manzo-Sánchez, Luis Carlos Salazar-Licea, Marco Tulio Buenrostro-Nava, Carlos L. Leopardi-Verde, Luciano Martínez-Bolaños, Ignacio Islas-Flores, Mario Orozco-Santos, Blondy Canto-Canché","doi":"10.1111/jph.13427","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Black Sigatoka disease is a significant threat to banana (<i>Musa</i> spp.) fruit yield and quality. For the control of the fungal pathogen, <i>Pseudocercospora fijiensis</i>, producers currently rely on fungicides such as Quinone outside Inhibitor (QoI). In this study, we examined the resistance status of <i>P. fijiensis</i> to QoI fungicides using 80 isolates from 24 localities in the main banana-producing areas of Mexico (Colima, Michoacán, Tabasco and Chiapas). Resistance was evaluated using the RFLP-PCR mutation assay on Cytochrome b gen (Cyt<i>b</i>). The results showed the G143A mutation in three isolates from Chiapas, indicating a relatively low mutation frequency in the sampled areas, where additionally, a microplate bioassay confirmed the resistance to fungicides. We also evaluated the genetic structure and differentiation among the sampled populations, detecting differences between populations within each region and among all populations. Furthermore, our analysis revealed shared haplotypes between resistant populations in Chiapas and nonresistant populations in Michoacán. These findings provide valuable insight into the resistance status of <i>P. fijiensis</i> to QoI fungicides in Mexico and serve as foundation for the development of strategies to manage strobilurin resistance in the country. Overall, this study highlights the importance of monitoring and implementing effective management practices to mitigate the spread of resistant strains.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of QoI-Resistant Isolates of the Banana Pathogen Pseudocercospora fijiensis in Mexico\",\"authors\":\"Gilberto Manzo-Sánchez, Luis Carlos Salazar-Licea, Marco Tulio Buenrostro-Nava, Carlos L. Leopardi-Verde, Luciano Martínez-Bolaños, Ignacio Islas-Flores, Mario Orozco-Santos, Blondy Canto-Canché\",\"doi\":\"10.1111/jph.13427\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Black Sigatoka disease is a significant threat to banana (<i>Musa</i> spp.) fruit yield and quality. For the control of the fungal pathogen, <i>Pseudocercospora fijiensis</i>, producers currently rely on fungicides such as Quinone outside Inhibitor (QoI). In this study, we examined the resistance status of <i>P. fijiensis</i> to QoI fungicides using 80 isolates from 24 localities in the main banana-producing areas of Mexico (Colima, Michoacán, Tabasco and Chiapas). Resistance was evaluated using the RFLP-PCR mutation assay on Cytochrome b gen (Cyt<i>b</i>). The results showed the G143A mutation in three isolates from Chiapas, indicating a relatively low mutation frequency in the sampled areas, where additionally, a microplate bioassay confirmed the resistance to fungicides. We also evaluated the genetic structure and differentiation among the sampled populations, detecting differences between populations within each region and among all populations. Furthermore, our analysis revealed shared haplotypes between resistant populations in Chiapas and nonresistant populations in Michoacán. These findings provide valuable insight into the resistance status of <i>P. fijiensis</i> to QoI fungicides in Mexico and serve as foundation for the development of strategies to manage strobilurin resistance in the country. Overall, this study highlights the importance of monitoring and implementing effective management practices to mitigate the spread of resistant strains.</p>\\n </div>\",\"PeriodicalId\":16843,\"journal\":{\"name\":\"Journal of Phytopathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jph.13427\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13427","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
黑斑病是香蕉(Musa spp.)果实产量和质量的一个重大威胁。为了控制真菌病原体 Pseudocercospora fijiensis,生产者目前依赖于醌外抑制剂(QoI)等杀菌剂。在这项研究中,我们利用来自墨西哥主要香蕉产区(科利马、米却肯、塔巴斯科和恰帕斯)24 个地方的 80 个分离物,考察了 P. fijiensis 对 QoI 杀菌剂的抗性状况。使用细胞色素 b 基因(Cytb)的 RFLP-PCR 突变检测法对抗药性进行了评估。结果显示,恰帕斯州的三个分离株出现了 G143A 突变,这表明取样地区的突变频率相对较低,此外,微孔板生物测定也证实了这些分离株对杀菌剂的抗性。我们还评估了取样种群的遗传结构和分化情况,发现每个地区的种群之间以及所有种群之间存在差异。此外,我们的分析还发现恰帕斯州的抗性种群与米却肯州的非抗性种群之间存在共同的单倍型。这些发现为深入了解墨西哥 P. fijiensis 对 QoI 杀菌剂的抗性状况提供了宝贵的信息,并为制定管理该国石硫合剂抗性的策略奠定了基础。总之,这项研究强调了监测和实施有效管理措施以减少抗性菌株传播的重要性。
Identification of QoI-Resistant Isolates of the Banana Pathogen Pseudocercospora fijiensis in Mexico
Black Sigatoka disease is a significant threat to banana (Musa spp.) fruit yield and quality. For the control of the fungal pathogen, Pseudocercospora fijiensis, producers currently rely on fungicides such as Quinone outside Inhibitor (QoI). In this study, we examined the resistance status of P. fijiensis to QoI fungicides using 80 isolates from 24 localities in the main banana-producing areas of Mexico (Colima, Michoacán, Tabasco and Chiapas). Resistance was evaluated using the RFLP-PCR mutation assay on Cytochrome b gen (Cytb). The results showed the G143A mutation in three isolates from Chiapas, indicating a relatively low mutation frequency in the sampled areas, where additionally, a microplate bioassay confirmed the resistance to fungicides. We also evaluated the genetic structure and differentiation among the sampled populations, detecting differences between populations within each region and among all populations. Furthermore, our analysis revealed shared haplotypes between resistant populations in Chiapas and nonresistant populations in Michoacán. These findings provide valuable insight into the resistance status of P. fijiensis to QoI fungicides in Mexico and serve as foundation for the development of strategies to manage strobilurin resistance in the country. Overall, this study highlights the importance of monitoring and implementing effective management practices to mitigate the spread of resistant strains.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.