{"title":"多巴脱羧酶在金刚夜蛾幼虫化蛹和免疫中的功能分析","authors":"Qiu-Li Hou , Han-Qiao Zhang , Jia-Ni Zhu , Er-Hu Chen","doi":"10.1016/j.pestbp.2024.106195","DOIUrl":null,"url":null,"abstract":"<div><div>The diamondback moth (<em>Plutella xylostella</em> L.), a notorious pest infesting cruciferous vegetables worldwide, has developed a high level of resistance to various commonly used chemical pesticides. In this paper, we explore whether dopa decarboxylase (DDC), which is essential for survival and development in insects, could be used as a potential target for the control of <em>P. xylostella</em>. Here, the full-length cDNA (<em>PxDDC</em>) of <em>P. xylostella</em> was identified, with a complete open reading frame of 1434 bp in length, encoding a protein of 477 amino acids. The temporal and spatial expression analysis showed a periodical expression pattern of <em>PxDDC</em> during molting, reaching a peak during the process of pupation, and it was found to be highly expressed in the epidermis of prepupal stage, indicating a crucial role of <em>PxDDC</em> in larval-pupal metamorphosis of <em>P. xylostella</em>. Subsequently, there was a significant decreasing in pupation and eclosion rates, and less production of melanin in <em>P. xylostella</em> after the disruption of <em>PxDDC</em> function by the injection of ds<em>PxDDC</em> (RNAi, RNA interference) or feeding a larval diet supplemented with L-α-methyl-DOPA (L-α-M-D) as DDC inhibitor. In addition, we found four antimicrobial peptide genes were significantly inhibited after feeding <em>P. xylostella</em> with L-α-M-D, and the injection of <em>Escherichia coli</em> could significantly increase insect mortality of enzyme inhibitor treated <em>P. xylostella</em>, suggesting <em>PxDDC</em> was involved in immune responses as well. In summary, these results confirm that <em>PxDDC</em> is required for larval-pupal metamorphosis and immunity of <em>P. xylostella</em>, suggesting a critical potential future novel insecticide target for RNAi based pest control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"206 ","pages":"Article 106195"},"PeriodicalIF":4.2000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functional analysis of dopa decarboxylase in the larval pupation and immunity of the diamondback moth, Plutella xylostella\",\"authors\":\"Qiu-Li Hou , Han-Qiao Zhang , Jia-Ni Zhu , Er-Hu Chen\",\"doi\":\"10.1016/j.pestbp.2024.106195\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The diamondback moth (<em>Plutella xylostella</em> L.), a notorious pest infesting cruciferous vegetables worldwide, has developed a high level of resistance to various commonly used chemical pesticides. In this paper, we explore whether dopa decarboxylase (DDC), which is essential for survival and development in insects, could be used as a potential target for the control of <em>P. xylostella</em>. Here, the full-length cDNA (<em>PxDDC</em>) of <em>P. xylostella</em> was identified, with a complete open reading frame of 1434 bp in length, encoding a protein of 477 amino acids. The temporal and spatial expression analysis showed a periodical expression pattern of <em>PxDDC</em> during molting, reaching a peak during the process of pupation, and it was found to be highly expressed in the epidermis of prepupal stage, indicating a crucial role of <em>PxDDC</em> in larval-pupal metamorphosis of <em>P. xylostella</em>. Subsequently, there was a significant decreasing in pupation and eclosion rates, and less production of melanin in <em>P. xylostella</em> after the disruption of <em>PxDDC</em> function by the injection of ds<em>PxDDC</em> (RNAi, RNA interference) or feeding a larval diet supplemented with L-α-methyl-DOPA (L-α-M-D) as DDC inhibitor. In addition, we found four antimicrobial peptide genes were significantly inhibited after feeding <em>P. xylostella</em> with L-α-M-D, and the injection of <em>Escherichia coli</em> could significantly increase insect mortality of enzyme inhibitor treated <em>P. xylostella</em>, suggesting <em>PxDDC</em> was involved in immune responses as well. In summary, these results confirm that <em>PxDDC</em> is required for larval-pupal metamorphosis and immunity of <em>P. xylostella</em>, suggesting a critical potential future novel insecticide target for RNAi based pest control.</div></div>\",\"PeriodicalId\":19828,\"journal\":{\"name\":\"Pesticide Biochemistry and Physiology\",\"volume\":\"206 \",\"pages\":\"Article 106195\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Biochemistry and Physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0048357524004280\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357524004280","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Functional analysis of dopa decarboxylase in the larval pupation and immunity of the diamondback moth, Plutella xylostella
The diamondback moth (Plutella xylostella L.), a notorious pest infesting cruciferous vegetables worldwide, has developed a high level of resistance to various commonly used chemical pesticides. In this paper, we explore whether dopa decarboxylase (DDC), which is essential for survival and development in insects, could be used as a potential target for the control of P. xylostella. Here, the full-length cDNA (PxDDC) of P. xylostella was identified, with a complete open reading frame of 1434 bp in length, encoding a protein of 477 amino acids. The temporal and spatial expression analysis showed a periodical expression pattern of PxDDC during molting, reaching a peak during the process of pupation, and it was found to be highly expressed in the epidermis of prepupal stage, indicating a crucial role of PxDDC in larval-pupal metamorphosis of P. xylostella. Subsequently, there was a significant decreasing in pupation and eclosion rates, and less production of melanin in P. xylostella after the disruption of PxDDC function by the injection of dsPxDDC (RNAi, RNA interference) or feeding a larval diet supplemented with L-α-methyl-DOPA (L-α-M-D) as DDC inhibitor. In addition, we found four antimicrobial peptide genes were significantly inhibited after feeding P. xylostella with L-α-M-D, and the injection of Escherichia coli could significantly increase insect mortality of enzyme inhibitor treated P. xylostella, suggesting PxDDC was involved in immune responses as well. In summary, these results confirm that PxDDC is required for larval-pupal metamorphosis and immunity of P. xylostella, suggesting a critical potential future novel insecticide target for RNAi based pest control.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.