同时存在多种 PML-RARA 异构体对急性早幼粒细胞白血病患者表型的影响

Emine Goktas, Ayse Gul Zamani, Mahmut Selman Yildirim, Sinan Demircioglu, Atakan Tekinalp, Ozcan Ceneli
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引用次数: 0

摘要

研究目的研究设计:横断面研究:横断面研究。研究地点和时间土耳其科尼亚内克梅廷-埃尔巴坎大学医学院医学遗传学系,2015年1月至2023年3月:研究组由确诊为 APL 的患者组成。通过采集血液或骨髓样本进行 RNA 分离,并使用实时 PCR 检测 PML-RARA bcr1、bcr2 和 bcr3 是否存在断点。然而,使用所使用的试剂盒无法提供 PML-RARA 融合转录本的定量结果:受检者中有 12 名女性和 8 名男性,平均年龄分别为 38 岁(19-80 岁)和 46.5 岁(22-60 岁)。根据同种异构体对患者进行评估时发现,40%的患者具有多种同种异构体。19名患者(95%)在治疗后获得了血液学缓解。只有一名有三种不同同工酶的患者没有得到缓解。单一异构体和多重异构体患者的估计中位生存期分别为 78.1 个月(95% CI:37.8-117.6)和 71.7 个月(46.2-97.2)。多同工酶型患者中有两人在早期死亡,而单一同工酶型患者没有早期死亡记录:结论:识别PML-RARA同工酶亚型对预测预后和临床随访具有重要意义:急性早幼粒细胞白血病 断点簇区 同工型 PML-RARA
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of Simultaneous Presence of Multiple PML-RARA Isoforms on Phenotype in Patients with Acute Promyelocytic Leukaemia.

Objective: To determine the coexistence of multiple PML-RARA transcripts in adult APL (acute promyelotic leukaemia) patients, and its impact on  the patients' laboratory parameters, treatment responses, and prognoses.

Study design: Cross-sectional study. Place and Duration of the Study: Department of Medical Genetics, Medical Faculty of Necmettin Erbakan University, Konya, Turkiye, from January 2015 to March 2023.

Methodology: The study group consisted of individuals diagnosed with APL. RNA isolation was performed by taking blood or bone marrow samples and the presence of breakpoints in PML-RARA bcr1, bcr2, and bcr3 was detected using the real-time PCR. However, the quantification of PML-RARA fusion transcripts cannot be provided using the utilised kit.

Results: Twelve women and eight men were examined with a mean age of 38 years (range: 19-80), and 46.5 years (range: 22-60) were examined, respectively. When evaluating patients based on isoforms, it was found that 40% had multiple isoforms. Nineteen (95%) patients achieved haematologic remission after the treatment. Only one patient who had three different isoforms did not achieve remission. The estimated median survival for patients with a single isoform and those with multiple isoforms was 78.1 months (95% CI: 37.8-117.6) and 71.7 months (46.2-97.2), respectively. Two of the patients with multiple isoforms were lost in the early stage, whereas no early-stage mortality was recorded among patients with a single isoform.

Conclusion: Identifying PML-RARA isoform subtypes is important for predicting prognosis and informing clinical follow-up.

Key words: Acute promyelocytic leukaemia, Breakpoint cluster region, Isoform, PML-RARA.

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