负载大蒜素的 ROS 响应水凝胶可抑制细胞凋亡，用于治疗大鼠模型中的椎间盘退变。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-26 DOI:10.1016/j.wneu.2024.10.056

Ting Zhang, Qing Huang, Liangjie Lu, Ke Zhou, Keqi Hu, Kaifeng Gan

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引用次数: 0

摘要

背景：椎间盘变性（IVDD）是导致下背痛的常见原因，而细胞凋亡在其进展过程中起着关键作用。本研究探讨了负载大蒜素的活性氧（ROS）反应性水凝胶治疗 IVDD 的潜力：方法：大蒜素被封装在ROS反应性水凝胶中，并在体外对其控制释放进行了研究。用过氧化氢处理核浆（NP）细胞以诱导其凋亡，并用 qPCR 和 Western 印迹法检测水凝胶的作用。此外，还建立了 IVDD 大鼠体内模型，以评估治疗效果：结果：ROS响应水凝胶通过降低ROS水平和调节凋亡和抗凋亡基因的表达，有效抑制了NP细胞的凋亡。在大鼠模型中，负载大蒜素的水凝胶显著减少了 IVDD，保持了椎间盘形态和基质的完整性：负载大蒜素的 ROS 响应水凝胶通过抑制细胞凋亡和减少体内椎间盘变性，显示出作为 IVDD 治疗方法的潜力。

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ROS-responsive hydrogel loaded with allicin suppresses cell apoptosis for the treatment of intervertebral disc degeneration in a rat model.

Background: Intervertebral disc degeneration (IVDD) is a common cause of lower back pain, and cell apoptosis plays a key role in its progression. This study explores the therapeutic potential of a reactive oxygen species (ROS)-responsive hydrogel loaded with allicin for treating IVDD.

Methods: Allicin was encapsulated in a ROS-responsive hydrogel, and its controlled release was studied in vitro. Nucleus pulposus (NP) cells were treated with hydrogen peroxide to induce apoptosis, and the effects of the hydrogel were examined using qPCR and Western blotting. An in vivo rat model of IVDD was also established to assess the efficacy of the treatment.

Results: The ROS-responsive hydrogel effectively inhibited apoptosis in NP cells by reducing ROS levels and modulating the expression of apoptotic and anti-apoptotic genes. In the rat model, the hydrogel loaded with allicin significantly reduced IVDD, preserving disc morphology and matrix integrity.

Conclusions: ROS-responsive hydrogel loaded with allicin shows potential as a therapeutic approach for IVDD by inhibiting cell apoptosis and reducing disc degeneration in vivo.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.