Wenji Cai, Min Wang, Amir Ei Hadad, Yuli Zhang, Simon D Tran, Samar Shurbaji, Gheyath K Nasrallah, Mariano Sanz, Sasha Omanovic, Faleh Tamimi
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Cell viability was assessed using live/dead assay and fluorescence microscopy.</p><p><strong>Results: </strong>Exposure of cells to high negative potentials caused cell detachment, while exposure to positive ones led to cell death on the cpTi surfaces. However, cellular viability was preserved when the electrical potentials were kept between -3 and +3 V. Cells retained 80% viability when subjected to -12.5 mA currents with an initial pOB cell count of 5 × 10<sup>4</sup>. However, when the initial cell count was elevated to 1 × 10<sup>5</sup>, the cells demonstrated the ability to withstand an even greater current (-25 mA) while preserving their vitality at the same level.</p><p><strong>Conclusion: </strong>Treatment of a titanium dental implant surface employing constant potential or current can harm cells surrounding dental implants. 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引用次数: 0
摘要
研究目的本研究旨在探讨采用恒定电流和电位对钛表面进行电化学处理对附着在表面上的组织细胞活力的影响，并确定这种处理方式的安全限度：方法：培养前成骨细胞（pOB）并将其播种到钛圆片上。然后使用连接到恒电位仪的三电极系统，将细胞播种的圆盘暴露在一系列等效直接电位（-6V-6V）或等效直接电流（-12.5 mA、-25 mA 或 -50 mA）下。使用活/死检测法和荧光显微镜评估细胞活力：结果：细胞暴露在高负电位下会导致细胞脱落，而暴露在正电位下会导致 cpTi 表面的细胞死亡。然而，当电位保持在 -3 至 +3 V 之间时，细胞的存活率得以保持。在初始 pOB 细胞数为 5 × 104 时，当电流为 -12.5 mA 时，细胞的存活率保持在 80%。然而，当初始细胞数增加到 1 × 105 时，细胞表现出了承受更大电流（-25 mA）的能力，同时保持了相同水平的活力：结论：使用恒定电位或电流处理钛金属牙科种植体表面会对牙科种植体周围的细胞造成伤害。然而，通过将电位控制在安全范围内，可以将这种损害降至最低。
The effect of titanium surface treatment by application of constant potential or current on the viability of pre-osteoblast cells: an in-vitro study.
Objectives: The aim of this study was to investigate the impact of electrochemical treatment of a titanium surface employing constant current and potential on the viability of the tissue cells attached to the surface and determining the safety limits for this type of treatment.
Methods: Pre-osteoblast cells (pOB) were cultured and seeded onto titanium discs. The cell-seeded discs were then exposed to a range of contant direct electrical potentials (-6V-6V) or contant direct electrical currents (-12.5 mA, -25 mA, or -50 mA) using a three-electrode system connected to a potentiostat. Cell viability was assessed using live/dead assay and fluorescence microscopy.
Results: Exposure of cells to high negative potentials caused cell detachment, while exposure to positive ones led to cell death on the cpTi surfaces. However, cellular viability was preserved when the electrical potentials were kept between -3 and +3 V. Cells retained 80% viability when subjected to -12.5 mA currents with an initial pOB cell count of 5 × 104. However, when the initial cell count was elevated to 1 × 105, the cells demonstrated the ability to withstand an even greater current (-25 mA) while preserving their vitality at the same level.
Conclusion: Treatment of a titanium dental implant surface employing constant potential or current can harm cells surrounding dental implants. However, this damage can be minimized by keeping the potential within a safety limit.
期刊介绍：
The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs.
In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.
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