Niels Plomp, Kristina Bertl, Marie-Louise Lydrup, Klas Sjöberg, Hermie J. M. Harmsen, Andreas Stavropoulos
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This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients.</p>\n </section>\n \n <section>\n \n <h3> Material and Methods</h3>\n \n <p>In a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. <i>Fusobacterium</i> was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of <i>Fusobacterium</i> with culturing, 16S rRNA gene profiling and a PCR-based approach.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In the first cohort, different <i>Fusobacterium</i> species were identified in GCF and saliva samples. However, as the total yield of <i>Fusobacterium</i> seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient-matched saliva and CRC resection material were screened for <i>Fusobacterium</i> and this showed that nine patients were culture-positive in the saliva samples; however, no <i>Fusobacterium</i> could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for <i>Fusobacterium</i>. All eight of these patients carried <i>Fusobacterium</i> in their saliva, indicated by both marker gene PCR and culture-based screening.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>These pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC-associated <i>Fusobacterium</i>, and a more in-depth analysis of specific strains and their characteristics in a larger cohort is justified.</p>\n </section>\n \n <section>\n \n <h3> Trial Registration</h3>\n \n <p>The protocol was registered at clinicaltrials.gov (NCT05945082).</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"10 6","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70016","citationCount":"0","resultStr":"{\"title\":\"Does Fusobacterium in Colorectal Cancer Sites Originate From the Oral Cavity? 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This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Material and Methods</h3>\\n \\n <p>In a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. <i>Fusobacterium</i> was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of <i>Fusobacterium</i> with culturing, 16S rRNA gene profiling and a PCR-based approach.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>In the first cohort, different <i>Fusobacterium</i> species were identified in GCF and saliva samples. However, as the total yield of <i>Fusobacterium</i> seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient-matched saliva and CRC resection material were screened for <i>Fusobacterium</i> and this showed that nine patients were culture-positive in the saliva samples; however, no <i>Fusobacterium</i> could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for <i>Fusobacterium</i>. All eight of these patients carried <i>Fusobacterium</i> in their saliva, indicated by both marker gene PCR and culture-based screening.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>These pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC-associated <i>Fusobacterium</i>, and a more in-depth analysis of specific strains and their characteristics in a larger cohort is justified.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Trial Registration</h3>\\n \\n <p>The protocol was registered at clinicaltrials.gov (NCT05945082).</p>\\n </section>\\n </div>\",\"PeriodicalId\":10203,\"journal\":{\"name\":\"Clinical and Experimental Dental Research\",\"volume\":\"10 6\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-11-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70016\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Dental Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70016\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Does Fusobacterium in Colorectal Cancer Sites Originate From the Oral Cavity? A Pilot Study
Objectives
Fusobacterium can contribute to oral diseases, but also pose as a systemic risk factor. This genus, and especially F. nucleatum, can be found in colorectal cancer (CRC) tissue and is involved in multiple aspects of this type of cancer. Previous studies indicated a possible oral origin of these bacteria; however, stronger evidence is needed to reach a definitive conclusion. This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients.
Material and Methods
In a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. Fusobacterium was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of Fusobacterium with culturing, 16S rRNA gene profiling and a PCR-based approach.
Results
In the first cohort, different Fusobacterium species were identified in GCF and saliva samples. However, as the total yield of Fusobacterium seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient-matched saliva and CRC resection material were screened for Fusobacterium and this showed that nine patients were culture-positive in the saliva samples; however, no Fusobacterium could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for Fusobacterium. All eight of these patients carried Fusobacterium in their saliva, indicated by both marker gene PCR and culture-based screening.
Conclusions
These pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC-associated Fusobacterium, and a more in-depth analysis of specific strains and their characteristics in a larger cohort is justified.
Trial Registration
The protocol was registered at clinicaltrials.gov (NCT05945082).
期刊介绍：
Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.
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