基于酪氨酸酶的毛细管流驱动微流控传感器,用于快速评估农药暴露情况,方便用户使用。

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Analyst Pub Date : 2024-11-04 DOI:10.1039/D4AN01203H
Claire E. Hefner, Prakash Aryal, Eric Brack, Todd Alexander and Charles S. Henry
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引用次数: 0

摘要

农药主要用于农业,以保护农作物并延长其寿命。然而,接触杀虫剂与各种急性和慢性健康影响有关,引起了人们对环境的极大关注。目前的检测方法往往依赖于复杂的仪器,既昂贵又耗时。虽然基于酶抑制的微流控纸质分析装置(mPAD)平台提供了一种更简便的替代方法,但它们存在分析物传输缓慢和分析物在微通道中吸附的问题。因此,我们需要一种快速、简单、经济高效的农药检测平台。在本研究中,我们提出了一种用于现场农药残留检测的快速微流控平台。与传统的 mPAD 平台不同,我们的系统可在几秒钟内通过中空毛细管通道传输农药样品,而不会在微通道中吸附农药。虽然许多研究都集中在纸张上的乙酰胆碱酯酶抑制上,但本研究是首次在纸张平台上引入基于酪氨酸酶抑制的农药检测方法。使用比色酶抑制测定法检测了一种具有代表性的二硫代氨基甲酸酯农药齐拉姆。检测限(LoD)为 1.5 ppm。在本研究中,我们对快速流动装置进行了优化,评估了其稳定性和对各种干扰的敏感性,并使用手套萃取法进行了实际样品测试,以评估其在实际环境中的能力。尖峰回收分析表明,皮手套的萃取效率为 82.5% 至 87.5%,丁腈手套的萃取效率为 68.9% 至 71.9%。该平台对干扰具有很强的选择性,在既定的储存方案下,酶的活性在一周后仍能保持 90%,还有进一步研究的空间。虽然这主要是一个概念验证,但该装置显示出作为农药检测额外工具的前景,未来有可能集成到多路复用装置中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Capillary-flow driven microfluidic sensor based on tyrosinase for fast user-friendly assessment of pesticide exposures†

Capillary-flow driven microfluidic sensor based on tyrosinase for fast user-friendly assessment of pesticide exposures†

Capillary-flow driven microfluidic sensor based on tyrosinase for fast user-friendly assessment of pesticide exposures†

Pesticides are primarily used in agriculture to protect crops and extend their longevity. However, pesticide exposure has been linked to various acute and chronic health effects, raising significant environmental concerns. Current detection methods are often expensive and time-consuming, relying on complex instruments. Although enzyme-inhibition-based microfluidic paper-based analytical device (mPAD) platforms offer an easier alternative, they suffer from slow analyte transport and analyte adsorption issues in microchannels. Consequently, there is a need for a fast, simple, and cost-effective point-of-need platform for pesticide sensing. In this study, we present a rapid microfluidic platform for on-site pesticide residue detection. Unlike traditional mPAD platforms, our system transports pesticide samples through hollow capillary channels within seconds without adsorption of pesticides in the microchannels. While much research has focused on acetylcholinesterase inhibition on paper, this study is the first to introduce a tyrosinase inhibition-based assay on a paper platform for pesticide detection. Ziram, a representative dithiocarbamate pesticide, was detected using a colorimetric enzymatic inhibition assay. A limit of detection (LoD) of 1.5 ppm was obtained. In this study, we optimized the fast-flow device, assessed its stability and susceptibility to various interferences, and conducted real-sample tests using glove extraction to evaluate its capability in real-world settings. Spike recovery analysis revealed an extraction efficiency of 82.5% to 87.5% for leather gloves and 68.9% to 71.9% for nitrile gloves. This platform demonstrates strong selectivity against interferences, with the enzyme retaining 90% activity even after a week under the established storage protocols with room for further investigation. While primarily a proof of concept, this device shows promise as an additional tool for pesticide detection, with potential future integration into multiplexed devices.

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来源期刊
Analyst
Analyst 化学-分析化学
CiteScore
7.80
自引率
4.80%
发文量
636
审稿时长
1.9 months
期刊介绍: "Analyst" journal is the home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences.
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