用于抗氧化和完整保存红细胞与血红蛋白的新型油封血点

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Genhan Zha , Xuan Xiao , Youli Tian , Hengying Zhu , Qiang Zhang , Changjie Yu , Honggen Li , Yuxing Wang , Chengxi Cao , Ping Chen
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引用次数: 0

摘要

传统 DBS 中的血红蛋白氧化 在过滤器上保存血红蛋白的可行性已得到验证,并在临床应用中被广泛接受。如图 1A 所示,传统的 DBS 保存血液样本时,在装血后 4 小时内,新鲜血液点的颜色会从鲜红色变为棕色,这表明铁血黄素(Fe2+)被氧化成了原铁血黄素(Fe3+)20。这种方法不仅能提供血红蛋白相对含量的信息,还能提供核酸数据和红细胞形态等重要细节,与传统的 DBS 相比,能提供更全面的样本信息。结论DBS 因其成本低、操作简单、运输方便等优点,已被广泛应用于生物医学研究和临床诊断中。然而,传统的 DBS 方法在干燥过程中会遇到防止血红蛋白氧化的难题,从而影响后续的诊断分析。我们提出了一种新颖的 BS-OS 血液保存方法,包括使用间隙隔离采样滤纸和在油封环境中冷藏。这种设计减少了Uncited reference[18];[19];[20];[21];[32];[33];[34];[35].Conflicts of interest作者声明没有与本文有竞争关系的经济利益。致谢本研究得到了广西科技基础与人才专项(编号:AD23026025)、中华人民共和国科学技术部(编号:KY202002008)、国家自然科学基金(编号:81703801、22104082、22074091、32175034)、上海交通大学 "交大之星 "计划医工交叉研究基金青年项目(批准号:24X010301289)的资助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A novel blood spot with oil sealing for antioxidation and completeness preservation of erythrocytes and hemoglobin

A novel blood spot with oil sealing for antioxidation and completeness preservation of erythrocytes and hemoglobin

A novel blood spot with oil sealing for antioxidation and completeness preservation of erythrocytes and hemoglobin
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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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