加拿大西部的 Dermacentor 种类(蛔虫:Ixodidae),并发现了 Dermacentor similis。

Grace K Nichol, Paula Lado, Louwrens P Snyman, Shaun J Dergousoff, J Scott Weese, Amy L Greer, Katie M Clow
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引用次数: 0

摘要

在加拿大,许多蜱类物种的分布范围正在显著扩大,其中包括几种Dermacentor spp Koch(Acari:Ixodidae)。最近在美国西部发现了 Dermacentor similis Lado,因此需要进行更多的研究来确定该物种目前的分布范围。研究人员从加拿大西部不列颠哥伦比亚省、阿尔伯塔省和萨斯喀彻温省的伴侣动物身上采集了 598 只蜱虫。对蜱虫进行了形态鉴定,然后对 ITS-2 部分基因目标(n = 595)进行 PCR 和凝胶电泳。97%(n = 579/595)的蜱产生了有效的条带模式。大多数(74%,n = 206/278)来自不列颠哥伦比亚省南部的 Dermacentor spp.的条带模式与 D. variabilis(Say)一致,而 26% (n = 72/278)与 D. andersoni Stiles 一致。在阿尔伯塔省的样本中,38%(n = 3/8)的带状模式与变异蜱一致,63%(n = 5/8)与安德森蜱一致。来自萨斯喀彻温省的所有(n = 293)蜱虫的带状模式与变异蜱一致。在 D. similis 的描述发表后,对线粒体(16S rDNA 基因、COI 基因)和核(ITS-2)标记进行了 DNA 测序,以确认 40 个样本的身份。有 27 个样本的条带模式与不列颠哥伦比亚省的 D. variabilis 一致,被确认为 D. similis。来自阿尔伯塔省的 1 个样本和来自萨斯喀彻温省的 5 个样本被确认为 D. variabilis,来自不列颠哥伦比亚省的 7 个样本被确认为 D. andersoni。ITS-2 扩增子对区分变种 D. 和 D. similis 没有帮助。这些结果提供了加拿大西部 D. similis 的证据,并强调线粒体基因序列可有效区分 D. andersoni、D. variabilis 和 D. similis。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dermacentor species (Acari: Ixodidae) in western Canada, with detection of Dermacentor similis.

Numerous tick species are undergoing significant range expansion in Canada, including several Dermacentor spp Koch (Acari: Ixodidae). With the recent description of Dermacentor similis Lado in the western United States, additional research is required to determine the current range of this species. Five hundred ninety-eight Dermacentor spp. were collected from companion animals in the western Canadian provinces of British Columbia, Alberta, and Saskatchewan. Ticks were morphologically identified to species, followed by PCR and gel electrophoresis of the ITS-2 partial gene target (n = 595). Ninety-seven percent (n = 579/595) generated valid banding patterns. The banding pattern for the majority (74%, n = 206/278) of Dermacentor spp. from southern British Columbia was consistent with D. variabilis (Say), while 26% (n = 72/278) was consistent with D. andersoni Stiles. For samples from Alberta, 38% (n = 3/8) had banding patterns consistent with D. variabilis and 63% (n = 5/8) with D. andersoni. All (n = 293) ticks from Saskatchewan had banding patterns consistent with D. variabilis. After the description of D. similis was published, DNA sequencing of mitochondrial (16S rDNA gene, COI gene) and nuclear (ITS-2) markers was used to confirm the identity of 40 samples. Twenty-seven samples that had banding patterns consistent with D. variabilis from British Columbia were confirmed to be D. similis. One sample from Alberta and five from Saskatchewan were confirmed to be D. variabilis and seven samples from British Columbia were D. andersoni. The ITS-2 amplicons were not useful for differentiating between D. variabilis and D. similis. These results provide evidence of D. similis in western Canada and highlight that sequences of the mitochondrial genes are effective for distinguishing D. andersoni, D. variabilis, and D. similis.

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