薏苡仁提取物对人视网膜色素上皮细胞炎症和纤维生成基因表达的影响

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2024-11-01 DOI:10.1016/j.biopha.2024.117646

Zahra Gharegezloo , Zahra Rezvani , Fatemeh Sanie-Jahromi , Foroogh Namjoyan

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引用次数: 0

摘要

增殖性玻璃体视网膜病变（PVR）是一种与视网膜脱落（RD）相关的威胁视力的疾病，主要由纤维细胞瘢痕膜形成引起。本研究探讨了阿德雷籽提取物馏分在减轻与视网膜色素上皮细胞（RPE）氧化应激、增殖、炎症和纤维形成相关的途径方面的治疗潜力。通过溶剂萃取获得阿德雷种子提取物馏分（甲醇：MeOH 和残留物：Res），并对其碳水化合物、蛋白质、类黄酮含量和抗氧化活性进行表征。培养 RPE 细胞，用 MTT 法评估它们对阿德雷萃取物的活力。用adlay馏分处理后，通过实时PCR对IL-1β、IL-6、LIF、TGF-β、Snail和α-SMA基因进行了基因表达分析。与 MeOH 萃取物相比，Res 萃取物中蛋白质、碳水化合物、黄酮类化合物和酚类物质的含量更高，抗氧化活性也明显增强。两种馏分都对 RPE 细胞的活力有抑制作用，其中 Res 馏分的影响更为明显。基因表达分析表明，MeOH馏分处理后，IL-6和TGF-β的表达明显下降，而Res馏分则导致IL-6、LIF、TGF-β、Snail和α-SMA的表达下降，表明对PVR相关通路的调节更为全面。这项研究强调了阿德雷种子提取物馏分在减轻 RPE 细胞中 PVR 相关通路方面的潜在治疗功效。Res萃取物富含生物活性化合物，具有很强的抗氧化活性，有望减轻氧化应激、增殖、炎症和纤维增生，这些都是PVR发生的关键过程。这些发现强调了阿德雷种子提取物作为一种新型 PVR 治疗策略的潜力，值得进一步研究和临床验证。

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The effect of Coix lachrymal L. seed extract on the expression of inflammation and fibrogenesis genes in human retinal pigment epithelial cells

Proliferative vitreoretinopathy (PVR) is a vision-threatening condition associated with retinal-detachment (RD), primarily caused by fibrocellular scar membrane formation. This study investigates the therapeutic potential of adlay seed extract fractions in mitigating PVR-associated pathways, focusing on oxidative stress, proliferation, inflammation, and fibrogenesis in retinal pigment epithelial (RPE) cells. Adlay seed extract fractions (methanolic: MeOH and residual: Res) were obtained through solvent extraction and characterized for carbohydrate, protein, flavonoid content, and antioxidant activity. RPE cells were cultured, and their viability in response to adlay fractions was assessed using the MTT assay. Gene expression analysis of IL-1β, IL-6, LIF, TGF-β, Snail and α-SMA genes was conducted via real-time PCR after treatment with adlay fractions. The Res fraction exhibited higher levels of protein, carbohydrate, flavonoids, and phenols compared to the MeOH fraction, along with significantly enhanced antioxidant activity. Both fractions showed inhibitory effects on RPE cell viability, with the Res fraction demonstrating a more pronounced impact. Gene expression analysis revealed a significant decrease in IL-6 and TGF-β expression with the MeOH fraction treatment, while the Res fraction led to decreased expression of IL-6, LIF, TGF-β, Snail and α-SMA, indicating a more comprehensive modulation of PVR-associated pathways. This study highlights the potential therapeutic benefits of adlay seed extract fractions in mitigating PVR-associated pathways in RPE cells. The Res fraction, particularly rich in bioactive compounds and exhibiting potent antioxidant activity, shows promise in attenuating oxidative stress, proliferation, inflammation, and fibrogenesis, critical processes in PVR development. These findings underscore the potential of adlay seed extracts as a novel therapeutic strategy for PVR warranting further investigation and clinical validation.

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.