中性和酸性枸杞多糖的理化特性和免疫调节活性比较研究

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2024-11-01 DOI:10.1016/j.biopha.2024.117659

Jia-Lun Duan , Meng-Qiu Liu , Ya-Nan Liu , Xiao-Fei Liang , Cheng Cao , An-Ni Yao , Li-Qiang Zhao , Sheng Guo , Da-Wei Qian , Chun-Jie Bao , Ming Zhao , Jin-Ao Duan

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引用次数: 0

摘要

枸杞多糖（LBPs）被认为是枸杞的主要生物活性成分，具有多种生物活性。然而，目前对枸杞多糖的研究主要局限于粗提取物，对其生物效应的结构特性了解有限。在本研究中，我们将枸杞多糖粗提物分为酸性枸杞多糖（ALBP）和中性枸杞多糖（NLBP），这两种枸杞多糖具有不同的理化性质。ALBP 含有 76.18% 的半乳糖醛酸（GalA），具有结晶性、热稳定性和胶状特性。相比之下，仅含有 3.16% GalA 的 NLBP 则显示出更多孔的结构和更优越的流动性。此外，功能分析表明，NLBP 通过激活树突状细胞和使巨噬细胞重新极化，增强了免疫调节作用。在携带 B16F10 黑色素瘤的 C57BL/6 J 小鼠模型中，NLBP 通过巨噬细胞再极化作用显著抑制了肿瘤的生长，抑制率高达 66.7%。研究结果凸显了 NLBP 和 ALBP 不同的生物效应，为枸杞多糖的精细利用提供了理论基础。

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Comparative study on physicochemical characterization and immunomodulatory activities of neutral and acidic Lycium barbarum polysaccharides

Lycium barbarum polysaccharides (LBPs) are recognized as key bioactive constituents of Lycium barbarum with diverse biological activities. However, current research on LBPs is largely confined to crude extracts, offering limited insight into the structural properties underlying their biological effects. In this study, we separated crude LBP into acidic LBP (ALBP) and neutral LBP (NLBP), which exhibited distinct physicochemical properties. ALBP, consisting of 76.18 % galacturonic acid (GalA), demonstrated crystallinity, thermal stability and gelatinous characteristics. In contrast, NLBP, with only 3.16 % GalA, displayed a more porous structure and superior fluidity. Furthermore, functional analysis revealed that NLBP exhibited enhanced immunoregulatory effects by activating dendritic cells and repolarizing macrophages. In a B16F10 melanoma-bearing C57BL/6 J mice model, NLBP significantly inhibited tumor growth with an inhibition rate of 66.7 % through macrophage repolarization. The findings highlight the distinct biological effects of NLBP and ALBP, providing a theoretical foundation for the refined utilization of LBP.

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.