纳米塑料对大脑皮层神经元多巴胺系统的影响

IF 1.2 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Na-Hyun Kim, Young-A Lee
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引用次数: 0

摘要

纳米塑料(NPx)可通过生态系统、吸入和皮肤接触进入生物体(包括人类),从肠道到大脑均可发现。然而,目前还不清楚 NPx 是否会累积并影响多巴胺系统。在这项研究中,我们在培养的小鼠大脑皮层神经元中研究了 NPx 对多巴胺系统的影响。用以下浓度的 100 nm NPx 处理培养的大脑皮层神经元 24 小时:1.896 × 105、3.791 × 106、7.583 × 107、1.571 × 109、3.033 × 1010 和 3.033 × 1011 粒子/毫升。多巴胺相关蛋白采用免疫荧光染色法进行分析。NPx以剂量依赖的方式诱导其在神经元中的积累,并增加多巴胺受体D1和D2的水平及其共表达。然而,NPx 处理并不影响神经元结构中其他多巴胺受体、多巴胺转运体、酪氨酸羟化酶、微管相关蛋白 2 或突触素的水平。这项研究表明,NPx 是一种潜在的多巴胺系统调节剂,通过其受体而不是其在神经元中的合成和再摄取来调节多巴胺系统,可能与以多巴胺为基础的精神疾病有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effects of Nanoplastics on the Dopamine System of Cerebrocortical Neurons.

Nanoplastics (NPx) can enter living organisms, including humans, through ecosystems, inhalation, and dermal contact and can be found from the intestine to the brain. However, it is unclear whether NPx accumulates and affects the dopamine system. In this study, we investigated the effects of NPx on the dopamine system in cultured murine cerebral cortex neurons. Cultured cerebrocortical neurons were treated with 100 nm NPx at the following concentrations for 24 h: 1.896 × 105, 3.791 × 106, 7.583 × 107, 1.571 × 109, 3.033 × 1010, and 3.033 × 1011 particles/mL. Dopamine-associated proteins were analyzed using immunofluorescence staining. NPx treatment induced its accumulation in neurons in a dose-dependent manner and increased the levels of dopamine receptors D1 and D2 and their co-expression. However, NPx treatment did not affect the levels of other dopamine receptors, dopamine transporters, tyrosine hydroxylase, and microtubule-associated protein 2, or synaptophysin in neuronal structures. This study demonstrated that NPx is a potential modulator of the dopamine system via its receptors rather than its synthesis and reuptake in neurons and may be associated with dopamine-based psychiatric disorders.

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来源期刊
CiteScore
3.40
自引率
4.50%
发文量
53
审稿时长
4.5 months
期刊介绍: The International Journal of Toxicology publishes timely, peer-reviewed papers on current topics important to toxicologists. Six bi-monthly issues cover a wide range of topics, including contemporary issues in toxicology, safety assessments, novel approaches to toxicological testing, mechanisms of toxicity, biomarkers, and risk assessment. The Journal also publishes invited reviews on contemporary topics, and features articles based on symposia. In addition, supplemental issues are routinely published on various special topics, including three supplements devoted to contributions from the Cosmetic Review Expert Panel.
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