解密花椰菜叶片的植物微生物组:通过对耐黑腐病基因型和易感基因型的元基因组和微生物学分析揭示花椰菜叶片微生物组。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Neelam Geat, Dinesh Singh, Partha Saha, Rajender Jatoth, Pedapudi Lokesh Babu, Gonur Somashekaraih Ramyashree Devi, Lalita Lakhran, Devendra Singh
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引用次数: 0

摘要

了解花椰菜植株中植物微生物组的动态,对于提高作物抵御野油菜黄单胞菌(Xanthomonas campestris pv. campestris)引起的黑腐病的能力具有重要意义。本研究调查了耐病(BR-161)和感病(Pusa Sharad)花椰菜基因型的可培养微生物组和元基因组概况,以阐明与耐病相关的微生物相互作用。从耐受性和易感性栽培品种的无症状叶片和黑腐病症状叶片中分离叶球细菌,获得了 46 种不同的细菌分离物。通过 16S rRNA 测序进行分子鉴定,发现不同基因型和健康状况下微生物类群的多样性存在差异。利用下一代测序技术进行的元基因组分析阐明了不同的微生物群落,在黑腐病症状叶片 BR-161 中观察到了更高的多样性。α和β多样性指数突显了不同基因型和健康状况下微生物群落结构和组成的差异。分类分析表明,在所有样本中,核心微生物群落由黄单胞菌属、精神杆菌属、乳杆菌属和假单胞菌属组成。通过微生物学方法验证,确认了这些关键菌属的存在。这些发现为了解耐黑腐病和易感黑腐病花椰菜基因型的植物微生物组提供了新的视角。利用本研究中发现的有益微生物群落为开发可持续的黑腐病管理策略、提高花椰菜作物的健康和产量提供了很好的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Deciphering Phyllomicrobiome of Cauliflower Leaf: Revelation by Metagenomic and Microbiological Analysis of Tolerant and Susceptible Genotypes Against Black Rot Disease.

Understanding the phyllomicrobiome dynamics in cauliflower plants holds significant promise for enhancing crop resilience against black rot disease, caused by Xanthomonas campestris pv. campestris. In this study, the culturable microbiome and metagenomic profile of tolerant (BR-161) and susceptible (Pusa Sharad) cauliflower genotypes were investigated to elucidate microbial interactions associated with disease tolerance. Isolation of phyllospheric bacteria from asymptomatic and black rot disease symptomatic leaves of tolerant and susceptible cultivars yielded 46 diverse bacterial isolates. Molecular identification via 16S rRNA sequencing revealed differences in the diversity of microbial taxa between genotypes and health conditions. Metagenomic profiling using next-generation sequencing elucidated distinct microbial communities, with higher diversity observed in black rot disease symptomatic leaf of BR-161. Alpha and beta diversity indices highlighted differences in microbial community structure and composition between genotypes and health conditions. Taxonomic analysis revealed a core microbiome consisting of genera such as Xanthomonas, Psychrobacillus, Lactobacillus, and Pseudomonas across all the samples. Validation through microbiological methods confirmed the presence of these key genera. The findings provide novel insights into the phyllomicrobiome of black rot-tolerant and susceptible genotypes of cauliflower. Harnessing beneficial microbial communities identified in this study offers promising avenues for developing sustainable strategies to manage black rot disease and enhance cauliflower crop health and productivity.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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