葡聚糖接枝聚丙烯酰胺/氧化锌纳米系统的毒性、抗菌、抗氧化、抗糖尿病和 DNA 裂解效应

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
M Serkan Yalçın, Sadin Özdemir, Volodymyr Prokopiuk, Pavlo Virych, Anatolii Onishchenko, Gülşah Tollu, Vadim Pavlenko, Nataliya Kutsevol, Nadir Dizge, Anton Tkachenko, Kasim Ocakoglu
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引用次数: 0

摘要

合成金属氧化物纳米颗粒-聚合物纳米复合材料是纳米技术中的一种新兴策略,可改善纳米颗粒的靶向递送并降低其毒性。在本研究中,我们报告了之前描述过的含葡聚糖接枝聚丙烯酰胺/氧化锌纳米颗粒(D-PAA/ZnO NPs)的混合纳米复合材料的生物效应,这些纳米复合材料由硫酸锌(D-PAA/ZnONPs(SO42-))和醋酸锌(D-PAA/ZnONPs(-OAc))制备而成,主要侧重于其抗菌活性。对 D-PAA/ZnONPs(SO42-)和 D-PAA/ZnONPs(-OAc)纳米系统进行了复合测试,以评估它们的抗氧化活性(DPPH 试验)、抗糖尿病潜力(α-淀粉酶抑制)、DNA 裂解活性、抗菌和抗生物膜活性。此外,还使用 MTT 法测试了 D-PAA/ZnONPs(SO42-) 和 D-PAA/ZnONPs(-OAc) 纳米系统对原代小鼠脾细胞的毒性。所研究的纳米系统抑制了大肠杆菌的生长。对于所有研究菌株,D-PAA/ZnONPs(SO42-) 和 D-PAA/ZnONPs(-OAc)的最低抑菌浓度(MICs)分别为 8 mg/L-128 mg/L 和 16 mg/L-128 mg/L。纳米复合材料的抗生物膜性能分别达到 94.27% 和 86.43%。这些化合物具有良好的抗氧化、抗α-淀粉酶和 DNA 裂解活性。D-PAA/ZnONPs(SO42-)和D-PAA/ZnONPs(-OAc)纳米系统可降低细胞存活率,并促进原代小鼠脾脏细胞死亡,其浓度高于抗菌浓度,表明存在治疗窗口。D-PAA/ZnONPs(SO42-)和D-PAA/ZnONPs(-OAc)纳米系统在良好的生物相容性背景下显示出抗氧化、抗糖尿病、DNA裂解、抗菌和抗生物膜活性,表明其具有治疗潜力,应在体内进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxicity, Antibacterial, Antioxidant, Antidiabetic, and DNA Cleavage Effects of Dextran-Graft-Polyacrylamide/Zinc Oxide Nanosystems.

Synthesis of metal oxide nanoparticles-polymer nanocomposites is an emerging strategy in nanotechnology to improve targeted delivery and reduce the toxicity of nanoparticles. In this study, we report biological effects of previously described hybrid nanocomposites containing dextran-graft-polyacrylamide/zinc oxide nanoparticles (D-PAA/ZnO NPs) prepared from zinc sulfate (D-PAA/ZnONPs(SO42-)) and zinc acetate (D-PAA/ZnONPs(-OAc)) focusing primarily on their antimicrobial activity. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems were tested in a complex way to assess their antioxidant activity (DPPH assay), antidiabetic potential (α-amylase inhibition), DNA cleavage activity, antimicrobial, and antibiofilm activity. In addition, the toxicity of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems against primary murine splenocytes was tested using MTT assay. The studied nanosystems inhibited E.coli growth. For all the investigated strains, minimum inhibitory concentrations (MICs) of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) were in the range of 8 mg/L-128 mg/L and 16 mg/L-128 mg/L, respectively. The nanocomposites demonstrated effective antibiofilm properties as 94.27% and 86.43%. The compounds showed good antioxidant, anti-α-amylase, and DNA cleavage activities. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems reduced cell viability and promoted cell death of primary murine spleen cells at concentrations higher than those that proved to be antibacterial indicating the presence of therapeutic window. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems show antioxidant, antidiabetic, DNA cleavage, antimicrobial, and antibiofilm activity against the background of good biocompatibility suggesting the presence of therapeutic potential, which should be further investigated in vivo.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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