Circ_0060927 通过疏导 miR-331-3p 和上调 TBX2 促进结直肠癌的发展

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-10-24 DOI:10.1016/j.prp.2024.155673

Dian Yin, XiaoLu Zhai, Xiu Feng, Mei Hua, Jing Liu, Ying Chen

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引用次数: 0

摘要

背景循环RNA（circRNA）的失调与结直肠癌（CRC）的发病机制密切相关。本研究旨在阐明 circ_0060927 在 CRC 中的生物学功能和作用机制。方法采用 5-乙炔基-2'-脱氧尿苷、细胞计数试剂盒-8（CCK-8）、流式细胞术和透孔试验以及异种移植肿瘤模型进行体外和体内分析。结果Circ_0060927的缺乏抑制了CRC细胞的增殖、自噬、迁移和侵袭，增加了细胞的凋亡和坏死，并抑制了肿瘤在体内的生长。Circ_0060927可与miR-331-3p结合，并通过海绵化miR-331-3p调控CRC细胞的行为。TBX2被miR-331-3p靶向，miR-331-3p靶向TBX2在CRC细胞中发挥抗癌作用。结论通过调节 miR-331-3p/TBX2 轴，下调 circ_0060927 可抑制 CRC 的进展，这可能为 CRC 提供了一个潜在的治疗靶点。

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Circ_0060927 promotes colorectal cancer development by sponging miR-331-3p and upregulating TBX2

Background

The dysregulation of circular RNAs (circRNAs) is closely associated with the pathogenesis of colorectal cancer (CRC). The present study aimed to elucidate the biological function and mechanism of circ_0060927 in CRC.

Methods

5-ethynyl-2’-deoxyuridine, Cell Counting Kit-8 (CCK-8), flow cytometry and transwell assays, as well as Xenograft tumor models were adopted for in vitro and in vivo analyses. The interaction between microRNA-331–3p (miR-331–3p) and circ_0060927 or T-box transcription factor 2 (TBX2) was verified by the dual-luciferase reporter and RNA pull-down assays.

Results

Circ_0060927 deficiency inhibited cell proliferation, autophagy, migration, and invasion and increased cell apoptosis and necrosis in CRC cells, as well as inhibited tumor growth in vivo. Circ_0060927 could bind to miR-331–3p, and circ_0060927 regulated CRC cell behaviors via sponging miR-331–3p. TBX2 was targeted by miR-331–3p, and miR-331–3p targeted TBX2 to exert the anti-cancer role in CRC cells. Mechanically, circ_0060927 regulated TBX2 expression by sequestering miR-331–3p in CRC cells.

Conclusion

Circ_0060927 downregulation inhibited CRC progression by regulating the miR-331–3p/TBX2 axis, which might offer a potential treatment target for CRC.

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.