用于分析应用的超极化台式 NMR 光谱仪

IF 7.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2024-11-01 DOI:10.1016/j.pnmrs.2024.10.001

Ana I. Silva Terra, Daniel A. Taylor, Meghan E. Halse

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引用次数: 0

摘要

台式 NMR 光谱仪具有中等磁场强度（B0=1-2.4T）和亚ppm 级的化学位移分辨率，是标准实验室 NMR（B0≥7T）的经济实惠且便携的替代品。然而，在转用低磁场仪器时，灵敏度和化学位移分辨率会显著降低。使用超极化技术可将台式 NMR 信号提高几个数量级，从而克服灵敏度限制。在目前可用的各种超极化方法中，动态核极化 (DNP)、对氢诱导极化 (PHIP) 和光化学诱导动态核极化 (photo-CIDNP) 是迄今为止最有希望与台式 NMR 集成用于分析应用的方法。在本综述中，我们总结了每种技术的理论，并讨论了如何将这些技术与台式 NMR 检测相结合的实例。我们讨论了将超极化台式 NMR 用于分析应用（从反应监测到生物分子相互作用探测）的进展，以及对未来的展望。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Hyperpolarised benchtop NMR spectroscopy for analytical applications

查看原文本刊更多论文

Hyperpolarised benchtop NMR spectroscopy for analytical applications

Benchtop NMR spectrometers, with moderate magnetic field strengths (

B_{0} = 1 - 2.4 T

) and sub-ppm chemical shift resolution, are an affordable and portable alternative to standard laboratory NMR (

B_{0} \geq 7 T

). However, in moving to lower magnetic field instruments, sensitivity and chemical shift resolution are significantly reduced. The sensitivity limitation can be overcome by using hyperpolarisation to boost benchtop NMR signals by orders of magnitude. Of the wide range of hyperpolarisation methods currently available, dynamic nuclear polarisation (DNP), parahydrogen-induced polarisation (PHIP) and photochemically-induced dynamic nuclear polarisation (photo-CIDNP) have, to date, shown the most promise for integration with benchtop NMR for analytical applications. In this review we provide a summary of the theory of each of these techniques and discuss examples of how they have been integrated with benchtop NMR detection. Progress towards the use of hyperpolarised benchtop NMR for analytical applications, ranging from reaction monitoring to probing biomolecular interactions, is discussed, along with perspectives for the future.

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来源期刊

Progress in Nuclear Magnetic Resonance Spectroscopy 物理-光谱学

CiteScore

14.30

自引率

8.20%

发文量

审稿时长

62 days

期刊介绍： Progress in Nuclear Magnetic Resonance Spectroscopy publishes review papers describing research related to the theory and application of NMR spectroscopy. This technique is widely applied in chemistry, physics, biochemistry and materials science, and also in many areas of biology and medicine. The journal publishes review articles covering applications in all of these and in related subjects, as well as in-depth treatments of the fundamental theory of and instrumental developments in NMR spectroscopy.