新出现的动物人类风险：在巴西的一只家犬中检测到人类诺罗病毒 GII.4_Sydney[P31] 株。

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2024-10-30 DOI:10.1016/j.actatropica.2024.107449

Lais Sampaio de Azevedo , Vanessa Cristina Martins Silva , Raquel Guiducci , Simone Guadagnucci , Fernanda Faria Costa , Monique Beerens Abdul Ghani , Ricardo Duarte Lopes , Antonio Charlys da Costa , Lia Cunha , Marcilio Figueredo Lemos , Adriana Parise , Regina Célia Moreira , Adriana Luchs

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引用次数: 0

摘要

最近人畜共患病的增加凸显了伴侣动物与城市环境的融合，带来了复杂的传播风险，突出了 "一体健康 "方法的必要性。呼吸道和肠道病毒一直与人类和动物之间的种间传播有关。本研究旨在评估巴西家犬和家猫中人类诺罗病毒（NoV）、腺病毒（HAdV）、肠道病毒（EV）、帕雷奇病毒（PeV-A）、博卡病毒（HBoV）、甲型肝炎病毒（HAV）和戊型肝炎病毒（HEV）、甲型和乙型流感病毒（Flu A/B）、呼吸道合胞病毒（RSV）以及 SARS-CoV-2 的传播情况，以了解潜在的动物传染病风险。2012 年至 2021 年间，巴西圣保罗州的小动物诊所收集了 600 份狗和猫的粪便样本（分别为 516 份和 84 份）。这些样本接受了 HBoV 和 HAdV 的内部 qPCR 筛查，同时使用内部 RT-qPCR 对 EV、PeV-A、NoV 和 HEV 进行了检测。SARS-CoV-2、Flu A/B 和 RSV 采用商用 RT-qPCR 试剂盒检测。HAV 检测采用传统的巢式 (RT)-PCR。对阳性样本进行了测序，以确定其分子特征并进行系统发育分析。在 0.2%（1/600）的动物中检测到了 NoV，而所有其他调查病毒的检测结果均为阴性。2012 年从一只宠物狗身上采集到的 NoV 阳性样本被鉴定为基因型 GII.4_Sydney[P31]。Dog/BRA/2012/GII.4_Sydney[P31]/IAL-M21株与巴西人类和环境中的NoV GII.4_Sydney[P31]株有密切的遗传关系，ORF1核苷酸相似度为98.1-99.2%，ORF2序列相似度为99.2-99.6%，表明存在种间传播。宠物狗经常接触到人类粪便传播的病毒，这凸显了由于宠物狗与人类在共同环境中的密切互动而造成动物间传播的可能性。目前迫切需要加强对伴侣动物的监测研究，以更好地了解在宠物体内检测到人类 NoV 株系的影响，因为 NoV 有可能在全球家庭、动物医院或收容所中成为反向人畜共患病。

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Emerging zooanthroponotic risks: Detection of the human norovirus GII.4 Sydney[P31] strain in a domestic dog in Brazil

Recent increases in zoonotic diseases underscore the integration of companion animals into urban environments, posing complex transmission risks and highlighting the necessity of One Health approaches. Respiratory and enteric viruses have been consistently linked to interspecies transmission between humans and animals. This study aimed to assess the circulation of human noroviruses (NoV), human adenoviruses (HAdV), enteroviruses (EV), parechoviruses (PeV-A), human bocaviruses (HBoV), hepatitis A (HAV) and E viruses (HEV), Influenza A and B viruses (Flu A/B), respiratory syncytial virus (RSV), and SARS-CoV-2 in domestic dogs and cats in Brazil to understand potential zooanthroponosis risks. Between 2012 and 2021, 600 fecal samples from dogs and cats (516 and 84, respectively) were collected at small animal clinics in São Paulo state, Brazil. The specimens underwent in-house qPCR screening for HBoV and HAdV, while EV, PeV-A, NoV, and HEV were tested using in-house RT-qPCR. SARS-CoV-2, Flu A/B, and RSV were investigated with a commercial RT-qPCR kit assay. HAV detection utilized conventional nested (RT)-PCR. Positive samples were sequenced for molecular characterization and phylogenetic analysis. NoV was detected in 0.2 % (1/600) of the animals, while all other investigated viruses tested negative. The NoV-positive sample, collected in 2012 from a pet dog, was identified as genotype GII.4_Sydney[P31]. The Dog/BRA/2012/GII.4_Sydney[P31]/IAL-M21 strain exhibited a close genetic relationship to Brazilian human and environmental NoV GII.4_Sydney[P31] strains, with 98.1–99.2 % nucleotide similarity in ORF1 and 99.2–99.6 % in ORF2 sequences, suggesting interspecies transmission. Pet dogs are frequently exposed to human fecal-borne viruses, highlighting the potential for zooanthroponotic transmission due to their close interaction with humans in shared environments. There is an urgent need to enhance surveillance studies in companion animals to better understand the implications of detecting human NoV strains in pets, as NoV could potentially act as a reverse zoonotic disease in households, animal hospitals, or shelters worldwide.

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.