Samson O. Egbewale , Ajit Kumar , Tosin A. Olasehinde , Mduduzi P. Mokoena , Ademola O. Olaniran
{"title":"ABTS 媒介物增强了嗜酸毛霉 FLU1 和嗜酸塔拉酵母菌 FLU12 的 Laccases 对荧蒽的生物转化作用","authors":"Samson O. Egbewale , Ajit Kumar , Tosin A. Olasehinde , Mduduzi P. Mokoena , Ademola O. Olaniran","doi":"10.1016/j.ibiod.2024.105946","DOIUrl":null,"url":null,"abstract":"<div><div>Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from <em>Trichoderma lixii</em> FLU1 (<em>Tl</em>FLU1L) and <em>Talaromyces pinophilus</em> FLU12 (<em>Tp</em>FLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. <em>Tl</em>FLU1L and <em>Tp</em>FLU12L exhibited almost similar <em>v</em><sub>max</sub> (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but <em>Tl</em>FLU1L showed a lower <em>K</em><sub>m</sub> as compared to <em>Tp</em>FLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased <em>v</em><sub>max</sub> to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased <em>K</em><sub>m</sub> to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, respectively. GC-MS analysis revealed that <em>Tl</em>FLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while <em>Tp</em>FLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (<em>Vibrio parahaemolyticus</em>) and HT22 cells. Thus, the study underscores the promising potential of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105946"},"PeriodicalIF":4.1000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ABTS mediator enhances biotransformation of fluoranthene by Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12\",\"authors\":\"Samson O. Egbewale , Ajit Kumar , Tosin A. Olasehinde , Mduduzi P. Mokoena , Ademola O. Olaniran\",\"doi\":\"10.1016/j.ibiod.2024.105946\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from <em>Trichoderma lixii</em> FLU1 (<em>Tl</em>FLU1L) and <em>Talaromyces pinophilus</em> FLU12 (<em>Tp</em>FLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. <em>Tl</em>FLU1L and <em>Tp</em>FLU12L exhibited almost similar <em>v</em><sub>max</sub> (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but <em>Tl</em>FLU1L showed a lower <em>K</em><sub>m</sub> as compared to <em>Tp</em>FLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased <em>v</em><sub>max</sub> to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased <em>K</em><sub>m</sub> to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, respectively. GC-MS analysis revealed that <em>Tl</em>FLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while <em>Tp</em>FLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (<em>Vibrio parahaemolyticus</em>) and HT22 cells. Thus, the study underscores the promising potential of <em>Tl</em>FLU1L and <em>Tp</em>FLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.</div></div>\",\"PeriodicalId\":13643,\"journal\":{\"name\":\"International Biodeterioration & Biodegradation\",\"volume\":\"196 \",\"pages\":\"Article 105946\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Biodeterioration & Biodegradation\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0964830524002178\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524002178","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
ABTS mediator enhances biotransformation of fluoranthene by Laccases from Trichoderma lixii FLU1 and Talaromyces pinophilus FLU12
Fluoranthene poses a significant environmental threat due to its persistence and toxicity. The Laccases from Trichoderma lixii FLU1 (TlFLU1L) and Talaromyces pinophilus FLU12 (TpFLU12L) are shown to act as biocatalysts for fluoranthene degradation. 3 U/mL of TlFLU1L and TpFLU12L reduced residual fluoranthene concentration to 49.5 ± 8.68 and 61.0 ± 5.66 %, while 10U/mL to 19.2 ± 5.95 and 28.7 ± 1.25 %, respectively, in 96 h. Mixing 200 μM ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with 3 U/mL enzyme in the reaction abolished 100 % residual fluoranthene within 48 h. TlFLU1L and TpFLU12L exhibited almost similar vmax (1.35 ± 0.02 and 1.29 ± 0.15 mg/L/h, respectively), but TlFLU1L showed a lower Km as compared to TpFLU12L (119.2 ± 0.02 and 170.8 ± 0.15 mg/L, respectively). ABTS significantly increased vmax to 7.73 ± 0.23 and 7.97 ± 0.28 mg/L/h, and decreased Km to 54.8 ± 0.27 and 26.6 ± 0.21 mg/L for TlFLU1L and TpFLU12L, respectively. GC-MS analysis revealed that TlFLU1L generated metabolites 9-oxo-fluorene-1-carboxylic acid, 9H-fluoren-9-one, and phthalic acid while TpFLU12L produced 9,10-phenanthrenedione and benzene-1,2,3-tricarboxylic acid. Ecotoxicity and cytotoxicity analysis of TlFLU1L and TpFLU12L degradation products in the presence of the mediator (ABTS) are found to be non-toxic towards marine bacteria (Vibrio parahaemolyticus) and HT22 cells. Thus, the study underscores the promising potential of TlFLU1L and TpFLU12L, particularly in conjunction with mediator (ABTS), for environment friendly and efficient bioremediation of fluoranthene-contaminated environments.
期刊介绍:
International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.