Caroline Ferreira de Santana , Isis Caroline da Silva Cirino , Larissa Isabela Oliveira de Souza , Marcia Vanusa da Silva , Daniela Maria do Amaral Ferraz Navarro , Júlio César Ribeiro de Oliveira Farias de Aguiar , Regina Celia Bressan de Queiroz Figueiredo , Ana Raquel Pereira da Silva , Clara Mariana Gonçalves Lima , Evandro G. da Silva Jr , Henrique Douglas Melo Coutinho , Tereza Cristina Leal-Balbino
{"title":"白芍药精油对耐多药鲍曼不动杆菌临床分离株的抗菌和细胞毒性作用","authors":"Caroline Ferreira de Santana , Isis Caroline da Silva Cirino , Larissa Isabela Oliveira de Souza , Marcia Vanusa da Silva , Daniela Maria do Amaral Ferraz Navarro , Júlio César Ribeiro de Oliveira Farias de Aguiar , Regina Celia Bressan de Queiroz Figueiredo , Ana Raquel Pereira da Silva , Clara Mariana Gonçalves Lima , Evandro G. da Silva Jr , Henrique Douglas Melo Coutinho , Tereza Cristina Leal-Balbino","doi":"10.1016/j.sajb.2024.10.019","DOIUrl":null,"url":null,"abstract":"<div><div>This study investigated the antimicrobial and cytotoxic effects of <em>Lippia alba</em> essential oil (LaEO) against five clinical isolates of multidrug-resistant <em>A. baumannii</em> from various infection sites in public hospitals in Recife, Pernambuco, Brazil. Gas chromatography-mass spectrometry analysis revealed 36 compounds in LaEO, with carvacrol (48.62 %), ρ-cymene (7.17 %), γ-terpinene (5.09 %), and thymol (5.00 %) as the most abundant constituents. LaEO exhibited potent inhibition of bacterial growth at MIC values ranging from 64 to 128 μg/mL, with MBC values similar to MIC. Additionally, interactions between LaEO and clinical antimicrobials (imipenem and gentamicin) demonstrated additive and synergistic effects when combined. Cytotoxicity assessment using the MTT assay on peritoneal macrophages revealed no significant difference in cell viability compared to the control, with a CC50 exceeding 512 μg/mL. Hemolytic activity on human red blood cells was minimal, with less than 2 % hemolysis observed at bacterially inhibitory concentrations.</div></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial and cytotoxic effects of Lippia alba essential oil on multidrug-resistant Acinetobacter baumannii clinical isolates\",\"authors\":\"Caroline Ferreira de Santana , Isis Caroline da Silva Cirino , Larissa Isabela Oliveira de Souza , Marcia Vanusa da Silva , Daniela Maria do Amaral Ferraz Navarro , Júlio César Ribeiro de Oliveira Farias de Aguiar , Regina Celia Bressan de Queiroz Figueiredo , Ana Raquel Pereira da Silva , Clara Mariana Gonçalves Lima , Evandro G. da Silva Jr , Henrique Douglas Melo Coutinho , Tereza Cristina Leal-Balbino\",\"doi\":\"10.1016/j.sajb.2024.10.019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study investigated the antimicrobial and cytotoxic effects of <em>Lippia alba</em> essential oil (LaEO) against five clinical isolates of multidrug-resistant <em>A. baumannii</em> from various infection sites in public hospitals in Recife, Pernambuco, Brazil. Gas chromatography-mass spectrometry analysis revealed 36 compounds in LaEO, with carvacrol (48.62 %), ρ-cymene (7.17 %), γ-terpinene (5.09 %), and thymol (5.00 %) as the most abundant constituents. LaEO exhibited potent inhibition of bacterial growth at MIC values ranging from 64 to 128 μg/mL, with MBC values similar to MIC. Additionally, interactions between LaEO and clinical antimicrobials (imipenem and gentamicin) demonstrated additive and synergistic effects when combined. Cytotoxicity assessment using the MTT assay on peritoneal macrophages revealed no significant difference in cell viability compared to the control, with a CC50 exceeding 512 μg/mL. Hemolytic activity on human red blood cells was minimal, with less than 2 % hemolysis observed at bacterially inhibitory concentrations.</div></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254629924006483\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254629924006483","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Antimicrobial and cytotoxic effects of Lippia alba essential oil on multidrug-resistant Acinetobacter baumannii clinical isolates
This study investigated the antimicrobial and cytotoxic effects of Lippia alba essential oil (LaEO) against five clinical isolates of multidrug-resistant A. baumannii from various infection sites in public hospitals in Recife, Pernambuco, Brazil. Gas chromatography-mass spectrometry analysis revealed 36 compounds in LaEO, with carvacrol (48.62 %), ρ-cymene (7.17 %), γ-terpinene (5.09 %), and thymol (5.00 %) as the most abundant constituents. LaEO exhibited potent inhibition of bacterial growth at MIC values ranging from 64 to 128 μg/mL, with MBC values similar to MIC. Additionally, interactions between LaEO and clinical antimicrobials (imipenem and gentamicin) demonstrated additive and synergistic effects when combined. Cytotoxicity assessment using the MTT assay on peritoneal macrophages revealed no significant difference in cell viability compared to the control, with a CC50 exceeding 512 μg/mL. Hemolytic activity on human red blood cells was minimal, with less than 2 % hemolysis observed at bacterially inhibitory concentrations.
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