感染玉米雷多菲诺病毒（MRFV）的玉米植株的生化化合物、化学元素和代谢物变化的综合分析

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2024-11-01 DOI:10.1016/j.pmpp.2024.102457

Marcos Vinicius Silva de Andrade , Magda Alana Pompelli Manica , Maria do Carmo Costa Silva , Roberto Venceslau de Carvalho , Rodolfo Vargas Castilhos , Antonio Gilberto Ferreira , Maria Cristina Canale

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引用次数: 0

摘要

玉米种植在全世界都至关重要，尤其是在拉丁美洲。然而，玉米矮缩病（corn stunt complex）相关病害对作物造成了严重影响，降低了谷物的产量和质量。玉米雷多菲诺病毒（MRFV）是这一复合病害的一部分，其症状的特点是沿叶脉出现萎黄小点，根据玉米基因型的不同，会出现发育不良并影响玉米棒的发育，从而导致生产力损失。然而，人们对这种错综复杂的植物-病原体关系还不甚了解。在这里，我们研究了由玉米叶蝉 Dalbulus maidis 传播的 MRFV 对受感染植株早期发育的影响。为了说明病毒感染对玉米植株的影响，我们进行了生化分析和核磁共振（NMR）分析。所有分析结果表明，与健康植株相比，受 MRFV 感染的玉米植株的化学和生化化合物以及代谢物的组成和活性都发生了变化。检测到超氧化物歧化酶、过氧化氢酶、过氧化物酶和β-葡聚糖酶的活性有所提高，而抗氧化活性和酚类化合物的变化较小。在化学成分方面，观察到了独特的变化，主要是钙（Ca）、磷（P）、钠（Na）和铁（Fe）等元素含量的增加。我们在玉米植株中发现了 20 种氨基酸、有机化合物和碳水化合物类代谢物，这些代谢物在 MRFV 感染期间受到调控。玉米植株通过调节酶和非酶活性以及化合物对麦地那龙线虫的食草行为做出了反应。这项研究深入探讨了玉米对 MRFV 的反应，并揭示了为实现可持续生产而寻找雷多菲诺病毒病抗性时应使用的参数。

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Comprehensive analysis of biochemical compounds, chemical elements and metabolites modifications in maize plants infected with maize rayado fino virus (MRFV)

Maize cultivation is crucial worldwide, especially in Latin America. However, diseases related to corn stunt complex have significantly impacted crops, reducing grain productivity and quality. Maize rayado fino virus (MRFV) is part of this complex, and its symptom is characterized by chlorotic dots along veins and, depending on maize genotype, there will be stunting and an impact on the corn cob development, which can lead to productivity losses. However, this intricate plant-pathogen relationship is yet not well understood. Here, we investigated the effects of MRFV transmitted by the corn leafhopper Dalbulus maidis in infected plants in their early stage of development. To show the impact of the viral infection in the maize plant, we performed biochemical and chemical analysis and nuclear magnetic resonance (NMR). The total set of analyses showed that changes in chemical and biochemical compounds, as well as in metabolites composition and activities, can be perceived in MRFV-infected maize plants when compared to healthy plants. An increase in the activity of superoxide dismutase, catalase, peroxidase, and β-glucanase was detected, whereas small changes have been identified in antioxidant activity and phenolic compounds. For chemical components, unique changes were observed, mainly the increase in the presence of some elements such as calcium (Ca), phosphorus (P), sodium (Na), and iron (Fe). We identified 20 metabolites of the amino acids, organic compounds, and carbohydrates classes in maize plants which are regulated during MRFV infection. Maize plants reacted to D. maidis herbivory by modulating both enzymatic and non-enzymatic activities, as well as chemical compounds. This research offers insights into the responses of maize against MRFV and sheds light on parameters to be used on the search of rayado fino viral disease resistance for a sustainable production.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.