表皮生长因子样结构域蛋白 6 预处理间充质干细胞提取的外泌体用于糖尿病伤口愈合

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2024-06-01 DOI:10.1016/j.reth.2024.09.009

Chen Gong , Chengde Xia , Linbo Liu

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引用次数: 0

摘要

糖尿病伤口在临床上难以有效修复。基于间充质干细胞（MSCs）的组织工程在伤口愈合方面显示出巨大的治疗潜力。间充质干细胞衍生的外泌体可将生物活性物质转移到受体细胞，从而再现间充质干细胞的功效。外泌体的生物功能取决于所衍生间充质干细胞的状态。在这项研究中，我们用EGFL6培养了hUC-间充质干细胞，并分离出EGFL6预处理外泌体（EGF-Exos），然后研究了EGF-Exos对伤口愈合的影响。结果表明，EGF-Exos能促进HUVECs的增殖和迁移，具有抗炎和改善血管生成的功能。此外，我们还制作了 Gelama 水凝胶来负载 EGF-Exos，以修复糖尿病伤口。体内实验结果表明，EGF-Exos有助于糖尿病伤口的修复，为了解EGF-Exos在糖尿病伤口愈合中的作用提供了宝贵的数据。

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Exosomes derived from epidermal growth factor-like domain protein 6-preconditioned mesenchymal stem cells for diabetic wound healing

Diabetic wounds are difficult to repair effectively in the clinic. Tissue engineering based on mesenchymal stem cells (MSCs) showed great therapeutic potential in wound healing. MSCs-derived exosome could reproduce the effect of MSCs by transferring the bioactive substance to the recipient cells. The biological function of exosomes was determined by the state of the derived MSCs. In this study, we cultured hUC-MSCs with EGFL6 and isolated EGFL6-preconditioned exosomes (EGF-Exos), and then investigated the effect of EGF-Exos on wound healing. The results revealed that EGF-Exos promoted the proliferation and migration of HUVECs, had the anti-inflammtory function and improved angiogenesis. Moreover, we fabricated Gelama hydrogel to load EGF-Exos to repair diabetic wounds. In vivo results showed that EGF-Exos contributed to the repair of diabetic wound and provided valuable data for understanding the role of EGF-Exos in diabetic wound healing.

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.