基于 D-π-A 结构的近红外开启荧光探针在小鼠肾缺血再灌注损伤中的半胱氨酸成像研究

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2024-10-28 DOI:10.1016/j.jphotochem.2024.116114

Chunpo Ge, Feng Pei, Pengcheng Zhang, Xiaoyu Wang, Xiaopeng Jiang, Kaiwen Chang, Zhijun Yang

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引用次数: 0

摘要

半胱氨酸（Cys）是一种重要的生物标志物，尤其适用于研究与氧化应激有关的生理和病理过程。检测 Cys 的小分子荧光探针在这些研究中至关重要。在这项研究中，我们利用二氰异佛尔酮和丙烯酸酯作为其结构成分，设计并合成了荧光探针 DCI-Th-Cys。DCI-Th-Cys 在 670 纳米波长处发出近红外荧光，与 Cys 反应后会产生 190 纳米波长的大斯托克斯位移，具有高灵敏度和高选择性。我们成功地在细胞和小鼠模型中对 Cys 进行了成像，特别是在肾缺血再灌注损伤小鼠模型的肾脏中检测到了 Cys 水平的升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Based on D–π–A structure of near-infrared turn-on fluorescent probe for cysteine imaging in renal ischemia–reperfusion injury in mice

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Based on D–π–A structure of near-infrared turn-on fluorescent probe for cysteine imaging in renal ischemia–reperfusion injury in mice

Cysteine (Cys) is an important biomarker, particularly relevant in studying physiological and pathological processes related to oxidative stress. Small-molecule fluorescent probes for detecting Cys are essential in these investigations. In this study, we designed and synthesized the fluorescent probe DCI-Th-Cys, utilizing dicyanoisophorone and acrylic ester as its structural components. DCI-Th-Cys exhibits near-infrared emission at 670 nm, with a large Stokes shift of 190 nm upon reacting with Cys, demonstrating high sensitivity and selectivity. We successfully imaged Cys in both cellular and mouse models, notably detecting elevated Cys levels in the kidneys of a mouse model with renal ischemia–reperfusion injury.

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.