Haydar Celik, Guven Akcay, Aysenur Budak Savas, Fatma Yesilyurt, Filiz Demirdogen, Dilanur Ates, Ahmet Hacimuftuoglu
{"title":"Trolox 可降低神经母细胞瘤细胞系诱导的氧化应激和炎症。","authors":"Haydar Celik, Guven Akcay, Aysenur Budak Savas, Fatma Yesilyurt, Filiz Demirdogen, Dilanur Ates, Ahmet Hacimuftuoglu","doi":"10.5137/1019-5149.JTN.46442-24.4","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line.</p><p><strong>Material and methods: </strong>SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 μM and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed.</p><p><strong>Results: </strong>Administration of 200 μM of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 μM of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results.</p><p><strong>Conclusion: </strong>The results of this experimental study demonstrated a Trolox concentration of 200 μM produces an anticancer effect on NB cell-line.</p>","PeriodicalId":94381,"journal":{"name":"Turkish neurosurgery","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Trolox Reduces Neuroblastoma-Induced Oxidative Stress and Inflammation.\",\"authors\":\"Haydar Celik, Guven Akcay, Aysenur Budak Savas, Fatma Yesilyurt, Filiz Demirdogen, Dilanur Ates, Ahmet Hacimuftuoglu\",\"doi\":\"10.5137/1019-5149.JTN.46442-24.4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line.</p><p><strong>Material and methods: </strong>SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 μM and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed.</p><p><strong>Results: </strong>Administration of 200 μM of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 μM of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results.</p><p><strong>Conclusion: </strong>The results of this experimental study demonstrated a Trolox concentration of 200 μM produces an anticancer effect on NB cell-line.</p>\",\"PeriodicalId\":94381,\"journal\":{\"name\":\"Turkish neurosurgery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish neurosurgery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5137/1019-5149.JTN.46442-24.4\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish neurosurgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5137/1019-5149.JTN.46442-24.4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Trolox Reduces Neuroblastoma-Induced Oxidative Stress and Inflammation.
Aim: To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line.
Material and methods: SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 μM and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed.
Results: Administration of 200 μM of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 μM of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results.
Conclusion: The results of this experimental study demonstrated a Trolox concentration of 200 μM produces an anticancer effect on NB cell-line.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
