{"title":"超越混合:同时检测和量化人类、狗和猫 DNA 的进展。","authors":"Hyeon-Jin Bae, Kyu-Sik Jeong, Jung-Eun Kim, Eun-Mi Hwang, Seong Yeon Yoo","doi":"10.1111/1556-4029.15644","DOIUrl":null,"url":null,"abstract":"<p><p>Animal-related crimes have increased with an increase in the number of pets worldwide, underscoring the importance of animal-related biological evidence at crime scenes. Evidence obtained in cases involving dogs and cats often includes a mixture of human and animal DNA. In this study, we developed a method using droplet digital polymerase chain reaction (ddPCR) to simultaneously identify and quantitatively detect human, dog, and cat DNA in mixed samples. HLA-DRA was chosen as a human-specific marker, OR6D7 as a dog-specific marker, and FLAI-K as a cat-specific marker. The species specificity of each target was confirmed using 14 control DNA samples from 11 mammals and 3 birds. Sensitivity tests determined the limit of detection to be 0.0008 ng/μL for human DNA and 0.00061 ng/μL for dog and cat DNA. In the mixture test, each DNA sample was independently and accurately detected in samples containing trace amounts of all three types of DNA. This study demonstrated the effectiveness of applying ddPCR to forensic case samples from dog- and cat-related incidents. We have presented a reliable method for the accurate identification and quantification of human, dog, and cat DNA simultaneously, offering possibilities for advancements in forensic DNA analysis and related fields.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Beyond the mix: Advancements in simultaneous detection and quantification of human, dog, and cat DNA.\",\"authors\":\"Hyeon-Jin Bae, Kyu-Sik Jeong, Jung-Eun Kim, Eun-Mi Hwang, Seong Yeon Yoo\",\"doi\":\"10.1111/1556-4029.15644\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Animal-related crimes have increased with an increase in the number of pets worldwide, underscoring the importance of animal-related biological evidence at crime scenes. Evidence obtained in cases involving dogs and cats often includes a mixture of human and animal DNA. In this study, we developed a method using droplet digital polymerase chain reaction (ddPCR) to simultaneously identify and quantitatively detect human, dog, and cat DNA in mixed samples. HLA-DRA was chosen as a human-specific marker, OR6D7 as a dog-specific marker, and FLAI-K as a cat-specific marker. The species specificity of each target was confirmed using 14 control DNA samples from 11 mammals and 3 birds. Sensitivity tests determined the limit of detection to be 0.0008 ng/μL for human DNA and 0.00061 ng/μL for dog and cat DNA. In the mixture test, each DNA sample was independently and accurately detected in samples containing trace amounts of all three types of DNA. This study demonstrated the effectiveness of applying ddPCR to forensic case samples from dog- and cat-related incidents. We have presented a reliable method for the accurate identification and quantification of human, dog, and cat DNA simultaneously, offering possibilities for advancements in forensic DNA analysis and related fields.</p>\",\"PeriodicalId\":94080,\"journal\":{\"name\":\"Journal of forensic sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of forensic sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/1556-4029.15644\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of forensic sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/1556-4029.15644","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
随着全球宠物数量的增加,与动物有关的犯罪也在增加,这凸显了犯罪现场与动物有关的生物证据的重要性。在涉及猫狗的案件中获得的证据通常包括人类和动物 DNA 的混合物。在这项研究中,我们开发了一种使用液滴数字聚合酶链反应(ddPCR)的方法,可同时鉴定和定量检测混合样本中的人、狗和猫的 DNA。我们选择 HLA-DRA 作为人的特异性标记,OR6D7 作为狗的特异性标记,FLAI-K 作为猫的特异性标记。使用来自 11 种哺乳动物和 3 种鸟类的 14 个对照 DNA 样本确认了每个目标的物种特异性。灵敏度测试表明,人类 DNA 的检测限为 0.0008 ng/μL,狗和猫 DNA 的检测限为 0.00061 ng/μL。在混合物测试中,每种 DNA 样品都能在含有微量三种 DNA 的样品中被独立、准确地检测出来。这项研究证明了将 ddPCR 应用于与狗和猫有关的法医案件样本的有效性。我们提出了一种同时准确鉴定和量化人、狗和猫 DNA 的可靠方法,为法医 DNA 分析及相关领域的发展提供了可能。
Beyond the mix: Advancements in simultaneous detection and quantification of human, dog, and cat DNA.
Animal-related crimes have increased with an increase in the number of pets worldwide, underscoring the importance of animal-related biological evidence at crime scenes. Evidence obtained in cases involving dogs and cats often includes a mixture of human and animal DNA. In this study, we developed a method using droplet digital polymerase chain reaction (ddPCR) to simultaneously identify and quantitatively detect human, dog, and cat DNA in mixed samples. HLA-DRA was chosen as a human-specific marker, OR6D7 as a dog-specific marker, and FLAI-K as a cat-specific marker. The species specificity of each target was confirmed using 14 control DNA samples from 11 mammals and 3 birds. Sensitivity tests determined the limit of detection to be 0.0008 ng/μL for human DNA and 0.00061 ng/μL for dog and cat DNA. In the mixture test, each DNA sample was independently and accurately detected in samples containing trace amounts of all three types of DNA. This study demonstrated the effectiveness of applying ddPCR to forensic case samples from dog- and cat-related incidents. We have presented a reliable method for the accurate identification and quantification of human, dog, and cat DNA simultaneously, offering possibilities for advancements in forensic DNA analysis and related fields.