南印度喀拉拉邦社区饲养的狗中支原体物种的分子检测和特征描述。

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina Pub Date : 2024-09-29 eCollection Date: 2024-09-01 DOI:10.17221/116/2023-VETMED

Gatchanda Shravan Kumar, Anju Varghese, Prabodh Kumar Hembram, Karapparambu Gopalan Ajith Kumar, Chundayil Kalarickal Deepa, Able Varghese, Reghu Ravindran

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引用次数: 0

摘要

血液支原体（血支原体）是一种强制性附红细胞体细菌，可感染多种脊椎动物宿主。印度以前从未尝试过对狗体内的支原体进行分子鉴定。因此，在本研究中，尝试使用 16S rRNA 基因对喀拉拉邦不同地区的犬支原体属进行分子鉴定。结果发现，M. haemocanis 的总体流行率为 18%。对所有选定序列进行的 NCBI-BLAST 分析表明，这些序列与 M. haemocanis 的序列相似度大于 99%。系统进化分析表明，M. haemocanis 和 M. haemofelis 聚为一个支系，表明遗传变异性较低。遗传距离数据和单倍型分析进一步证实了这一点。

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Molecular detection and characterisation of Mycoplasma species in community owned dogs of Kerala, a South Indian State.

Haemotropic mycoplasmas (haemoplasmas) are obligate epierythrocytic bacteria that infect a wide range of vertebrate hosts. The molecular characterisation of Mycoplasma organisms in dogs has not previously been attempted from India. Hence, in the present study, the molecular characterisation of Mycoplasma spp. in dogs of different zones of Kerala was attempted using 16S rRNA gene. An overall prevalence of 18% for M. haemocanis was detected. The NCBI-BLAST analysis of all the selected sequences revealed > 99% identity with the sequences of M. haemocanis. The phylogenetic analysis revealed clustering of M. haemocanis and M. haemofelis in a single clade indicating low genetic variability. It was further supported by the genetic distance data and haplotype analysis.

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来源期刊

Veterinarni Medicina Veterinary Sciences-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.