传染性法氏囊病病毒 VP5 的单克隆抗体开发和抗原表位鉴定。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal Pub Date : 2024-10-29 DOI:10.1016/j.tvjl.2024.106254

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引用次数: 0

摘要

传染性法氏囊病（IBD）是一种重要的鸡免疫抑制疾病，由传染性法氏囊病病毒（IBDV）感染引起。VP5 是 IBDV 复制的非必需蛋白，但在 IBDV 发病机制中起着关键作用。目前还缺乏对 VP5 生物功能的深入了解。本研究利用原核系统表达和纯化了 IBDV 优势流行株的可溶性 VP5，并开发了一种能分泌 IBDV VP5 单克隆抗体（MAb）的杂交瘤细胞系。VP5 MAb 对 IBDV VP5 有很强的特异性，能有效区分 IBDV 及其 VP5 缺失株。此外，还鉴定出了 IBDV VP5 的抗原表位 137RRDLPKPE145，这是首次详细报道 IBDV VP5 抗原表位。该抗原表位位于 VP5 的 C 端，在不同的 IBDV 血清 1 型毒株中具有一致性。这项研究的发现为进一步探索基因功能和 VP5 的差异检测提供了宝贵的见解。

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Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5

Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5-deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.

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来源期刊

Veterinary journal 农林科学-兽医学

CiteScore

4.10

自引率

4.50%

发文量

审稿时长

40 days

期刊介绍： The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.