地塞米松和阿奇霉素通过调节脂质代谢提高山羊精子保存质量

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2024-10-26 DOI:10.1016/j.theriogenology.2024.10.025

Yongjie Xu , Shixin Sun , Mingyue Wang , Wenzheng Shen , Lei Wang , Chunhuan Ren , Yinghui Ling , Zijun Zhang , Hongguo Cao

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引用次数: 0

摘要

山羊精液中的磷脂酶A（PLA）会与精液稀释液中的蛋黄聚集，导致精子死亡。本研究旨在解决磷脂酶A与蛋黄相互作用导致精子死亡的问题，并探讨地塞米松（DXMS）和阿奇霉素（AZM）在低温条件下对山羊精子的保护作用和代谢调节机制。在4 °C的低温条件下，向含有30 μg/mL AZM的精液稀释液中添加不同浓度的地塞米松，以检测山羊精子的质量。经测定，DXMS 的最佳浓度为 20 μg/mL。在储存的第5天，对20 μg/mL DXMS组的精子进行了抗氧化能力、总胆固醇（TC）水平、能量代谢和代谢组学分析。结果表明，在 4 °C下，含有 30 μg/mL AZM 的组中，聚乳酸和蛋黄之间的相互作用不会导致精子聚集。20 μg/mL DXMS 显著提高了精子活力、质膜完整性、顶体完整性、谷胱甘肽过氧化物酶（GPX）（P 2+ 显著下降（P

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Dexamethasone and azithromycin enhance goat sperm preservation quality by regulating lipid metabolism

Phospholipase A (PLA) in goat semen aggregates with egg yolk in semen diluent, leading to sperm death. The aim of this study is to address the issue of sperm death caused by the interaction between PLA and egg yolk, and to explore the protective effect and metabolic regulation mechanism of the combination of dexamethasone (DXMS) and azithromycin (AZM) on goat sperm under low temperature conditions. At a low temperature of 4 °C, different concentrations of DXMS were added to semen diluents containing 30 μg/mL AZM to detect the quality of goat sperm. The optimal concentration of DXMS was determined to be 20 μg/mL. On the 5th day of storage, antioxidant capacity, total cholesterol (TC) levels, energy metabolism, and metabolomics analysis were performed on the sperm of the 20 μg/mL DXMS group. The results showed that there was no aggregation caused by the interaction between PLA and egg yolk in the group containing 30 μg/mL AZM at 4 °C. 20 μg/mL DXMS significantly improved sperm motility, plasma membrane integrity, acrosome integrity, glutathione peroxidase (GPX) (P < 0.05), catalase (CAT) (P < 0.01), and superoxide dismutase (SOD) activity (P < 0.01). The content of reactive oxygen species (ROS) and Fe²⁺ significantly decreased (P < 0.01), while the content of ATP (P < 0.01) and TC (P < 0.05) significantly increased. Through metabolomics analysis, a total of 56 differential metabolites (P < 0.05) were screened, including 5a, 6-Anhydrotetracycline, Betamethasone, and 11-Dehydrocorticosterone, mainly enriched in 8 metabolic pathways (P < 0.05), including steroid hormone biosynthesis, glycerophospholipid metabolism, and choline metabolism in cancer. Among them, 5 metabolic pathways are related to lipid metabolism. The results indicate that AZM effectively inhibits the aggregation of PLA and yolk, and the combination of AZM and DXMS enhances the preservation quality of goat sperm during low-temperature preservation by regulating lipid metabolism.

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.