利用基于 CRISPR/Cas9 的基因打靶技术生成 SFTPC-mCherry 基因敲入报告人胚胎干细胞 WAe001-A-2H。

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-10-28 DOI:10.1016/j.scr.2024.103597

Hui Sun , Qian Li , Tao Xu , Wei Zhang , Jiaqi Sun , Huisheng Liu

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引用次数: 0

摘要

SFTPC基因负责产生肺表面活性蛋白C（SPC），这是一种高度疏水性分子，通过影响肺泡表面活性蛋白的合成，在维持肺完整性方面发挥着至关重要的作用。在这项研究中，我们利用CRISPR/Cas9系统进行了精确的基因组编辑，创建了一个修饰的H1人类胚胎干细胞（hESC）系，其中包含了SFTPC-mCherry报告基因构建体。因此，工程化的 SFTPC-mCherry 基因敲入（KI）hESC 株可以作为一种有效的工具，在肺泡 2 型细胞从 hESCs 分化过程中跟踪 SFTPC 基因的表达模式。

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Generation of SFTPC-mCherry knock-in reporter human embryonic stem cell line, WAe001-A-2H, using CRISPR/Cas9-based gene targeting

The SFTPC gene is responsible for the production of the pulmonary surfactant protein C (SPC), a highly hydrophobic molecule that plays a crucial role in maintaining lung integrity through its influence on the synthesis of alveolar surfactant proteins. In this study, we harnessed the CRISPR/Cas9 system for precise genome editing to create a modified H1 human embryonic stem cell (hESC) line, incorporating the SFTPC-mCherry reporter construct. Therefore, the engineered SFTPC-mCherry knock-in (KI) hESC line can serve as an effective tool for tracking the expression patterns of the SFTPC gene as alveolar type 2 cells differentiate from hESCs.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.