番茄 K+ 转运体 SlHAK5 的突变会改变花的雌蕊形态、离子组、代谢组和转录组。

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Jesús Amo, Elisa Jiménez-Estévez, Almudena Martínez-Martínez, Adrián Yáñez, Vicente Martínez, Manuel Nieves-Cordones, Francisco Rubio
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引用次数: 0

摘要

植物组织中的 K+ 积累是植物生长和发育的关键因素。番茄高亲和性 K+转运体 SlHAK5 对于根部从低浓度外界环境中获取 K+至关重要。它还参与花粉中 K+ 的积累和植物的繁殖力,因为 slhak5 KO 植物表现出花粉萌发率低、花粉管生长受阻和果实孤雌生殖。在这里,我们对 slhak5 花进行了深入分析,结果显示其在解剖学、离子组学、代谢组学和转录组学水平上存在相关缺陷。首先,slhak5花表现出较短的花柱和增大的子房,再加上slhak5 X WT杂交果实中种子数量较少,表明slhak5突变对雌性生育能力有影响。其次,与野生型相比,突变体花中 Ca2+ 以及氨基酸、柠檬酸和糖等几种代谢物的积累较低,而吲哚-3-乙酸的含量则有所增加。第三,对野生型和 slhak5 植物的雌蕊和雄蕊进行的 RNAseq 分析显示,在雄蕊的基因表达分析中,运输和信号通路显著富集。因此,可以得出结论,需要功能性 SlHAK5 转运体来维持花朵中适当的 Ca2+、代谢物和基因表达水平,缺失 SlHAK5 转运体会导致雄性和雌性生育能力严重下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mutation of the K+ transporter SlHAK5 of tomato alters pistil morphology, ionome, metabolome and transcriptome in flowers.

K+ accumulation in plant tissues is a crucial factor for plant growth and development. The tomato high-affinity K+ transporter SlHAK5 is essential for root K+ acquisition from low external concentrations. It is also involved in K+ accumulation in pollen and plant fertility as slhak5 KO plants show a low rate of pollen germination, impaired pollen tube growth and parthenocarpic fruits. Here, we present a thorough analysis of slhak5 flowers, which showed relevant defects at the anatomic, ionomic, metabolomic and transcriptomic levels. First, slhak5 flowers exhibited shorter styles and enlarged ovaries that, together with a low number of seeds in fruits from slhak5 X WT crosses, indicated an effect of the slhak5 mutation on female fertility. Second, a lower accumulation of Ca2+, as well as of several metabolites such as amino acids, citric acid and sugars, was observed in mutant flowers, whereas indole-3-acetic acid content was increased when compared to the wild-type. Third, RNAseq conducted on pistils and stamens of wild-type and slhak5 plants revealed that transport and signalling pathways are significantly enriched in the gene expression analyses of stamens. Thus, it can be concluded that a functional SlHAK5 transporter is required to maintain appropriate Ca2+, metabolite and gene expression levels in flowers, and its absence leads to important reductions in both male and female fertility.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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